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Information × Registration Number 0216U006711, 0111U008741 , R & D reports Title Light-dependent hydrogen production by microalgae (Chlorophyta) and photosynthetic bacteria (Rhodobacter) popup.stage_title Head Zolotareva Elena Kostantinovna, Registration Date 28-04-2016 Organization M.G. Kholodny Institute of Botany NASU popup.description2 Report on research: 34 p. 5 Fig. 3 tab., 30 references. The objects of research were microalgae isolated from natural reservoirs in Ukraine, as well as algolog pure cultures of Euglena gracilis and Chlamydomonas reinhardtii. The objective was to analyze the processed data, to develop scientific foundations of hydrogen generation by microalgae, determining the maximum duration of the process, favorable light conditions and and terms of optimal nutrition. Methods: microbiological, biochemical, physical - chemical and spectral. In 2015 we completed studies of the effect of exogenous factors (in particular, the trace amounts of zinc, vanadate and nickel) on the efficiency of hydrogen generation by Euglena gracilis and Chlamydomonas reinhardtii. To do this, the required amounts of microalgae were grown by varying culture conditions.A further purification of the original strains of anaerobic purple photosynthetic bacteria, in particular, Rubrivivax gelatinosus, isolated from natural soil samples from different ecological conditions was performed. Determination of bioenergetic processes in cells of E. gracilis as the basis for conversion of solar energy into the molecular hydrogen was continued. Stimulation of cellular respiration with the addition of exogenous substrates and efficiency of photosynthetic energy transformation in E. gracilis in anoxic conditions was studied, that allowed the development of microalgae cultivation conditions with anoxic cell state. The contribution of chloroplast respiration into anoxia state formation and induction of the process of photohydrogen evolution in E. gracilis cells. Under certain conditions, culture can exist and produce hydrogen without loss of long-term viability. The recommendations for the use of microalgae for H2 production in the process of photosynthesis were elaborated based on the findings. Product Description popup.authors Мокросноп Вікторія Михайлівна Подорванов Володимир Вікторович Поліщук Олександр Васильович Степанов Сергій Степанович Шнюкова Єлізавета Іллівна popup.nrat_date 2020-04-02 Close