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Information × Registration Number 0217U000058, 0115U001378 , R & D reports Title Identification of tumor-associated microRNAs as predictive circulating breast cancer markers popup.stage_title Head Chekhun Vasyl Fedorovych, Registration Date 16-01-2017 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 Expression profiles of miRNAs, responsible for the development of resistance (miR-200b, -34a), invasion (miR-10b, -320a), apoptosis (miR-221, -133, -122) and hormonal status (miR-29b) were studied in cell human breast cancer lines with different sensitivity to cytotoxic drugs. It was established that the development of resistance to cytostatic drugs is accompanied by a significant reduction in oncosuppressive miR-200b and -320a in breast cancer cells resistant to doxorubicin (up to 0,08 ± 0,007 cu and 0,37 ± 0,042 cu, respectively) and in breast cancer cells resistant to cisplatin (up to 0,19 ± 0,004 cu and 0,74 ± 0,025 cu, respectively). It was shown that the development of resistance to cisplatin and doxorubicin is accompanied by increased expression of oncogenic miR-221 to 17,1 ± 0,39 cu and 23,68 ± 0,57 cu, respectively. Similar changes of studied microRNAs profile were identified in tumor tissue of Walker 256 carcinosarcoma, resistant to doxorubicin. Along with this, Walker 256 carcinosarcoma tumors resistant to doxorubicin had decreased levels of miR-122 and -133, responsible for glucose and iron metabolism of 1.5 and 3.6 times, respectively. Thus, a study found that tumor cells with low sensitivity to cytostatic drugs are characterized by increased levels of expression of oncogenic miRNAs (miR-10b, -221), reduction in oncosuppressive microRNA (miR-122, -133a) oncosuppressive and microRNAs that regulate protein responsible for sensitivity to anticancer drugs (miR-200b, -34a). Theese findings are a fundamental basis for microRNAs studies in tumors of patients with breast cancer. Product Description popup.authors Борікун Тетяна Вікторівна Лук'янова Наталія Юріївна Чехун Василь Федорович popup.nrat_date 2020-04-02 Close