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Information × Registration Number 0217U004177, 0114U003034 , R & D reports Title To develop a manufacturing technology of antigen preparations for the diagnosis of rickettsial diseases in Ukraine and for epidemiological surveillance of them popup.stage_title Head Kushnir Zenon, Registration Date 19-01-2017 Organization Lviv State Research Institute of Epidemiology & Hygiene popup.description2 The goal of the work - improving the technological development process of antigenic preparations from strains pathogenic rickettsia and epidemiological research activity of existing foci rickettsial infections. Object of study - rickettsia strains, rickettsial antigens, rickettsiosis, typhus, Q fever, tick-borne spotted fever, pediculosis. Research methods: rickettsiological, microbiological, serological, molecular genetic, epidemiological, entomological, statistics. Devices - pH-meter, weights, colorimeter. Analyzed the modern epidemic situation of rickettsial infections and shown the relevance of development domestic antigenic preparations for laboratory diagnosis of these diseases. Studied the technological process of manufacturing rickettsial antigenic preparations, modern methodological principles learned from studying the biological activity of pathogenic strains of rickettsiae using polymerase chain reaction in real time. Conducted researches on guinea pigs strains pathogenic rickettsiae indicated they saved, but a reduced biological activity after prolonged storage at low temperature. It outlines the appropriate stage of the technological process in the manufacture of experimental models rickettsial antigenic preparations, namely enhancement of reproductive cells sensitive laboratory models to produce the necessary antigenic biomass. The received new data on the epidemic situation in Ukraine with typhus, including the preservation of the pathogen in the vector populations (lice) in different regions of Ukraine that confirmed by modern methods of PCR and Immunofluorescent microscopy. According to the study Ixodes ticks and murine rodents found Q fever pathogen circulation in natural foci of infection in Odesa and Ivano-Frankivsk regions. Detection of acute disease and recent transferred diseases among residents of Odesa region confirmed the further development of the epidemic process Q fever in these areas. By results of researches published scientific papers, published by Ukrmedpatentinform declarative patent, three newsletters, declarative patent, prepared six information-analytical reviews that are embedded in health care facilities. Socio-economic efficiency of work is determined by timely detection of people diseases on rickettsiosis and prevention of epidemic outbreaks in the population. Product Description popup.authors Бек Наталя Георгіївна Зарічна Ольга Зіновіївна Логінов Юрій Олександрович Луцик Тетяна Семенівна Топорович Оксана Іванівна Чіпак Наталя Іванівна popup.nrat_date 2020-04-02 Close