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Registration Number

0218U002464, 0113U000226 , R & D reports

Title

Reproductive biology characteristics, structural and functional adaptations of tropical and subtropical plants under glasshouse conditions and in in vitro culture

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Head

Buyun Lyudmyla Ivanivna,

Registration Date

15-03-2018

Organization

M.M.Grishko National Botanical Garden of Ukrainian Academy of Science

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Research report: 1 vol., 309 p.; 96 figures; 23 tables; 329 references. Object of study - biomorphology, comparative anatomy and morphology, peculiarities of reproduction systems of tropical plants from Amaryllidaceae (Phaedranassa Herb.), Asparagaceae (Asparagus Tour. ex L., Sansevieria Thunb.), Asphodelaceae (Aloe L.), Begoniaceae (Begonia L.), Orchidaceae (Coelogyne Lindl., Cymbidium Sw., Paphiopedilum Pfitz.), Theaceae (Camellia L.), Rutaceae (Citrus L.) families under glasshouse conditions and in in vitro culture. Subject matter - structural organization of generative and vegetative systems, breeding systems, surface micromorphology of seeds and leaves, methods of reproduction both in vivo and iv vitro, orchid pollen cryopreservation, design of exhibitions for conservation of tropical plants biodiversity in situ and ex situ. Goal of investigations - to assess the peculiarities of plant reproduction and and structural and functional adaptations of tropical plants both under glasshouse conditions and in in vitro culture aimed at effective ex situ conservation of tropical plants biodiversity. Investigation methods - plant introduction, phenological observations, comparative morphology and anatomy, light and scanning electron (SEM) microscopy, biotechnological, high-performance liquid chromatography (HPLC), anatomical and stomatographical, statistical methods. The diversity of shoots and shoot systems characteristics of plants belonging to various families within Asparagales order: Coelogyne (Orchidaceae), Sansevieria (Asparagaceae) and Camellia L. (Theaceae) as well was assessed. The peculiarities of various reproduction strategies within various angiosperm families, e.g. Begoniaceae (Begonia), Orchidaceae (Coelogyne, Cymbidium, Paphiopedilum) were evaluated. The morphological, anatomical, physiological and biochemical peculiarities of plant adaptation in different experimental models (in vivo and in vitro) (on the examples of orchid plant genera Coelogyne and Paphiopedilum (Orchidaceae) have been found out. At first time the possibilities of long-time preservation of orchid pollinaria at - 18?С have been revealed. The distinctive micromorphological patterns of leaf surface of tropical orchids both juvenile seedlings propagated in in vitro culture and adult plants of Coelogyne viscosa Lindl. (Orchidaceae) cultivated in glasshouses have been exhibited. It was shown that epidermal structure patterns can be used as biological markers for assessing adaptation potential of orchid plants and predicting the acclimatization success of in vitro propagated seedlings to ex vitro conditions. For the first time, the chromatographic profiles of orchid seed endometabolites as well as alellopathic activities of 10 orchid species belonging to different ecological groups have been investigated from the viewpoint of the orchid-mycobiont relationships. KEYWARDS: ex situ biodiversity conservation, reproduction systems both in vivo and in vitro, structural adaptation, biomorphology, micromorphology, biotechnology, exhibition glasshouses.

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Іванніков Роман Вікторович
Іваннікова Наталія Станіславівна
Бєлаєва Яна Володимирівна
Буюн Людмила Іванівна
Вахрушкін Володимир Семенович
Войченко Юрій Оникійович
Гиренко Олександр Григорович
Головко Раїса Петрівна
Гурненко Іван Володимирович
Жила Алла Іванівна
Кикавська Наталія Іванівна
Кикавський Ігорь Вікторович
Ковальська Людмила Африканівна
Краснєнкова Ірина Леонідівна
Мальцов Ігор Юрійович
Опришко Марина Валеріївна
Харитонова Ірина Прокопівна
Харченко Ігорь Іванович
Черевченко Тетяна Михайлівна
Ярославська Жанна Миколаївна

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2020-04-02