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0218U003593,
0115U004198
,
Improvement of gene delivery efficiency into plants by using novel nanoscale polycationic polymers
Stoika Rostyslav,
18-04-2018
Institute of cell biology, National Academy of Sciences of Ukraine
It was shown the low level of investigated polymers cytotoxicity for callus cells of Arabidopsis thaliana and the ability of polymers to transfer plasmid DNA into the Arabidopsis thaliana callus cells, which are surrounded by a secondary cell wall. Polymers are capable of delivering plasmid DNA in protoplasts of moss Ceratodon rurpureus. The PCR method confirmed that the transformants of C. purpureus transformants have the desired construct. Polymers do not possess genotoxic and mutagenic activity (it has not been revealed the ability of polymeric carriers to induce the appearance of gene mutations in the Ames test and to increase in the frequency of chromosomal aberrations and microcurrents in Allium cepa ).
Ключівська Ольга Юріївна
Фінюк Наталія Степанівна
2020-04-02
Updated: 2025-12-08
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