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Information × Registration Number 0218U005036, 0117U003926 , R & D reports Title Molecular genetic and morphological features of of lower limb tissues regeneration under conditions of chronic hyperglycemia popup.stage_title Head Dubovyk Yevhen, Registration Date 07-02-2018 Organization Sumy State University popup.description2 As a result of the implementation of phase I, a clinical database for the representatives of the comparison groups was created, taking into account demographic, anthropometric, clinical indicators and information about the results of laboratory and instrumental examination. The biological material (venous blood) from 163 patients with type 2 diabetes mellitus and 110 controls individuals was collected and DNA was extracted. Forward and reverse primer structures, amplification regimes and restriction enzymes for locus rs1044498 (gene ENPP1), rs699946 (VEGF-A gene) and rs1800247 (BGLAP) was selected. At the same time the method of chronic hyperglycemia modeling in rats was performed using a two-week load of 10% aqueous solution of fructose followed by a single injection of streptozotocin. Product Description popup.authors Гарбузова Є.А. Годовас Д.В. Дубовик Є.І. Колбунова В.С. Літовченко Б.М. Максимова О.С. Марченко І.В. Матлай О.І. Олешко Т.Б. Пастухова К.О. Сухонос О.В. Тимошенко О.О. Фоменко І.Г. Шапоревський Є.О. popup.nrat_date 2020-04-02 Close