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Information × Registration Number 0218U005072, 0115U002314 , R & D reports Title Biocenotic basis and molecular genetic mechanisms of cultivation of biological resistant highly productive forests popup.stage_title Head Krynytskyy H.T., Registration Date 19-02-2018 Organization National Forestry University of Ukraine popup.description2 The objects of the study are middle-aged, ripening, ripe and overstretched oak-pine, pine-beech and beech stands, as well as pine trees of all ages with symptoms of root rot and bacteriosis, seeds and juvenile pine plants. The goal of the study is to work out the biocenotic and genetic principles of the introduction of nature-approximate forestry in Ukraine, to find out the molecular mechanisms of the immune response of Scots pine, the main forest-forming breed of Ukraine, to the action of phytopathogens and to determine the prospects for using pine resistance genes as markers for estimating tree genotypes for resistance to diseases. Methods of research - forestry, forest assesment, microbiological, molecular biological, genetic engineering, bioinformatic, statistical. The biocenotic concept of introduction of nature-approximate forestry in Ukraine was presented in the paper. The processes of natural renewal of wood species, methods and mechanisms of reforming the same-age tree stands into different age-old forests were covered. The differential expression profiles of the defensin genes in pine seedling tissues on response to pathogen with different life-style were revealed. The molecular cloning of the Scots pine defensin gene 1 was performed and its intron-exon structure was established. The sequence of this gene was deposited in the GenBank database under number KH 690107. The molecular genetic methods of diagnosing pathogens of root rot, fusariosis and bacterial dropsy of pine trees have been developed, that allow identification of the pathogens on the early stages of infection. Product Description popup.authors Груник Наталія Ігорівна Гут Роман Тарасович Данчук Олег Тадейович Дебринюк Юрій Михайлович Ковальова Валентина Андріївна Крамарець Володимир Олександрович Криницька Ольга Григорівна Криницький Григорій Томкович Кузів Роман Федорович Скольський Ігор Михайлович Чернявський Микола Васильович Шаловило Юлія Ігорівна Юсипович Юрій Михайлович popup.nrat_date 2020-04-02 Close