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Information × Registration Number 0219U001104, 0117U006124 , R & D reports Title Role of epithelial-mesenchymal transition in mechanisms of drug resistance formation in prostate cancer cells popup.stage_title Head Lykhova Olexandra Olexandrivna, Registration Date 14-01-2019 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 We conducted the study of the possibility of using exogenous LF as an EMТ modifier in human cells with different sensitivity to hormonal therapy. We determined variously directed changes in the expression of miR-200b and -199a in hormone-sensitive and hormpne-refractory prostate cancer cells, namely, a decrease in their level of 0.57 and 0.27 times, respectively, in the LNCaP line and increase in their levels in the DU-145 line is 4.45 and 2.07 times, respectively after LF treatment. It was established that under the influence of exogenous LF in the cells of both studied lines, there is an increase in the level of miR-21, accompanied by a decrease in the expression of ? and progesterone estrogen receptors. It was found that the cultivation of hormone-sensitive and hormone-refractory PC cells with exogenous LF does not cause changes in the expression of N-cadherin, vimentin, actin and pancitokeratin. It is shown that under the influence of exogenous LF in cells of the hormone-refractory line DU-145 demonstrated an increase in the expression of E-cadherin in 5.3 times. Exogenous LF has been shown to reduce the proliferative and invasive activity of DU-145 and LNCaP cell lines by 41% and 50%, and 40% and 30%, respectively. Product Description popup.authors Борікун Тетяна Вікторівна Горбик Дарія Миколаївна Задворний Тарас Володимирович Лихова Олександра Олександрівна popup.nrat_date 2020-04-02 Close