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Information × Registration Number 0219U001211, 0115U001360 , R & D reports Title The mechanisms of the degradation of peroxisomal and cytosolic proteins in methylotrophic yeast and creation of the overprodusers of proteins with commercial value from mutants with damage of these mechanisms popup.stage_title Head Sibirny Andriy Andriyovych, Доктор біологічних наук Registration Date 18-01-2019 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The decreasing of the specific activity of fructose-1,6-bisphosphatase in the wild type strain GS200, the strain with the deletion of the GSS1 hexose sensor gene and strain defected in autophagy pathway SMD1163 of P. pastoris in short-term and long-term induction with methanol, and with or without the addition of the MG132 (proteasome degradation inhibitor) was investigated. Degradation of fructose-1,6-bisphosphatase by the Western blot analysis in GS200, SMD1163 and ?gss1 strains was studied. Conclusions. It was shown that the duration of cell incubation on methanol has no particular effect on the inactivation of enzymes. The effect of the proteasome inhibitor MG132 was insignificant. Catabolic inactivation of cytosolic and peroxisomal enzymes is damaged in the ?gss1 mutant as glucose signaling is impaired. Fructose-1,6-bisphosphatase degrades by a vacuolar pathway, regardless of the duration of methanol induction, which correlates with the activity data of this enzyme.To confirm FBP degradation pathway the recombinant strains with GFP-labeled Fbp1 of K. phaffii (or E. coli) and RFP-labeled peroxisomes were constructed on the background of GS200, pex3? and SMD1163. The fluorescent microscopy analysis of the constructed strains is under the progress54815481 Product Description popup.authors Дмитрук Олена Валеріївна Семків Марта Віталіївна Сибірний Андрій Андрійович popup.nrat_date 2020-04-02 Close