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Information × Registration Number 0219U004963, 0115U002934 , R & D reports Title Testing of carbon-protein constructs for targeted delivery of anticancer drugs. popup.stage_title Head Sydorenko Myhail V., Registration Date 20-12-2019 Organization "Department of Biotechnical Problems in Diagnostic of Institute of Problems of Cryobiology and Cryomedicine National Academy of Sciences of Ukraine" popup.description2 Abstract. Research report: 30 pp., 29 sources of cited literature. Object of study - tumor cells of colon cancer carcinoma HT29, 2D and 3D cell models in vitro, model of laboratory mice Balb2 / b - in vivo, multilayer carbon nanotubes. The goal is to evaluate the possibilities of using CNTs and their derivatives, namely BHT-Doc, in antitumor therapy. Research methods - histological, cytomorphological, microscopic, hematology. As a result of the work, a carbon compound was obtained, functionalization of an antitumor drug and a fluorescent label. In a monolayer culture, oxidized CNTs and BHT-Doc have a moderate cytotoxic effect at low concentrations (12.5 ?g/ml). With an increase in concentration to 25-50-100 ?g / ml, the viability of tumor cells decreases dose-dependently. With an increase in the concentration of CNTs to 200 ?g / ml, the viability of NT29 decreased to 39.2% for BHTox and 50% for BHT-Doc. In a spheroidal culture of tumor cells, it was shown that oxidized CNTs can stimulate the formation of BPS by almost 10 times. BHT-Doc stimulate the formation of BPS by 2.83 times. In most experiments, BHT-Doc has no cytotoxic effect on tumor cells in both 2D and 3D culture, which could be compared with the effect of a single doxorubicin. The simultaneous use of low concentrations of trypsin and BHT-Doc significantly increases the cytotoxic effect of CNTs and Doxorubicin compared to using these substances separately. When studying the effects of BHTox and BHT-Doc in vivo, the greatest effect was found on hepatic enzymes. In hematological studies, signs of hematological toxicity of CNT-Doc and pure Doxurubicin were revealed. What finds expression in dose-dependent myelosuppression, leukopenia and neutropenia (neutropenia). With the introduction of CNT-Doc, pathological tissue remodeling of the liver is determined. Thus, based on the results obtained, we assume that the carbon-protein construct (BHT-Doc) is able to carry the drug in the cell microenvironment but does not cause significant cytotoxic effects. In the presence of peptidase (trypsin), doxorubicin is released and its cytotoxic effect increases. The indicated effect was observed in experiments both in vitro and in vivo. Key words: colon cancer carcinoma tumor cells, stem tumor cells, multicellular tumor spheroids, doxorubicin, multilayer carbon nanotubes. Product Description popup.authors Гергелюк Тетяна Сергіївна Перепелиціна Олена Михайлівна Сидоренко Михайло Васильвич Ястребова Олена Вікторівна popup.nrat_date 2020-04-02 Close