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Information × Registration Number 0219U005629, 0113U007276 , R & D reports Title Exploration of the role of mitochondria in responses of cardiovascular system at different functional states of an organism popup.stage_title Head Sagach Vadym Fedorovych, Registration Date 26-12-2019 Organization Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine popup.description2 The aim of the work was to investigate the role of signaling compounds of sulfur in the reactions of the cardiovascular system in different patologies. It has been shown that endogenous hydrogen sulfide synthesis (H2S) is sharply reduced in aging, experimental diabetes, hypertension that is accompanied by deterioration of endothelial-dependent vascular relaxation and can be greatly enhanced by stimulation of H2S synthesis. For the first time, it has been shown that the course introduction of the cofactor of H2S-synthesis enzymes pyridoxal-5 phosphate restored the synthesis of hydrogen sulfide in the heart at aging, suppressed Ca2+ -induced opening of mitochondrial permeability transition pores, reduces oxidative-nitrosatitative stress, contributed to the restoration of the NO synthesis and contractile function of the heart of the old animals after ischemia-reperfusion. It has been revealed that mitochondrial H2S regulates calcium homeostasis in mitochondria since the inhibitor of MPST leded to deterioration of mitochondrial breathing, decrease of calcium accumulation, increase of sensitivity to Ca2+ as inducer of MPTP. Inhibition of mitochondrial H2S leads to the reduced ability of the myocardium to develop the growth of contractile function in response to volume load. For the first time we showed the role of H2S in the regulation of the heart function in heterometric regulation and in the provision of central blood circulation regulation has been established, since the blockade of H2S synthesis enzymes (CBS, MPST) was accompanied by the absence of a hypotensive effect from the introduction of the L-cysteine ??substrate into the nucleus of the dorsomedial division of the oblongata. In experiments on isolated mitochondria, it has been shown that the activation of mitochondrial potassium ATP-dependent channels by its pharmacological agents (diazoxide and pinacidyl) reduces the ability of mitochondria to produce ROS, which is the main mechanism induced by the cardioprotective cascades. The features of calcium channels in the endothelium under genetically determined atherosclerosis were aslo investigated. It has been established that dysfunction of IK Ca channels in endothelial cells can be the cause of genetically determined atherosclerosis. Product Description popup.authors І.Ю.Охай А.І.Бондаренко А.Ю.Лучкова Б.С. Коп"як В.Ф. Сагач К.О.Драчук Л.А.Мись Л.М. Шаповал Н.А. Струтинська Н.О. Дорофєєва О.В. Акопова О.В.Дмитренко Р.А.Федічкіна Т.А. Ільїна Ю.В.Гошовська Ю.П.Коркач popup.nrat_date 2020-04-02 Close