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Information × Registration Number 0219U102194, 0119U103201 , R & D reports Title Assessment of the genetic polymorphism of Colobanthus quitensis (Kunth) Bartl. in the Antarctic by analyzing the structure of the genes of high-conserved cytoskeleton proteins popup.stage_title Head Blume Yaroslav B., Доктор біологічних наук Registration Date 20-12-2019 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2 The detailed molecular genetic analysis of C. quitensis natural populations was carried out using four DNA marker systems to detect intron length polymorphism of cytoskeletal protein genes. A low level of C. quitensis genetic polymorphism was detected. Among the studied populations, the population from the Booth Island is significantly different, which may be a consequence of changes in the conditions of species existence. This results can be used to determine a factors that affect to the C. quitensis genetic variation in the Antarctic. Assessment of 1st and 2nd intron length polymorphism of β-tubulin genes demonstrated that TBP method can be effectively used as a DNA marker system for differentiating Antarctic pearl ecotypes. A single genotype was isolated within Skua Island population using сТВР method. The assessment of the α- and γ-tubulin intron length polymorphism didn’t allow genotyping and differentiation of C. quitensis populations among themselves. In addition no differences between C. quitensis populations were found using intron length polymorphism of actin genes. However, the data obtained indicate the possibility of further use of this DNA marker system for conducting Antarctic pearl molecular genetic analysis. Overall, the research results indicate the feasibility of using DNA markers, which are based on the analysis of the structure of the highly conserved cytoskeleton protein genes, for molecular genetic analysis of C. quitensis populations. Moreover, the method of assessing the intron length polymorphism of β-tubulin genes was shown as the most sensitive. In the future, TBP method will be effectively use for fingerprinting various Antarctic pearl ecotypes.   Product Description popup.authors Bilonozhko Yuliya O. Blume Yaroslav B. Pirko Yaroslav V. Postovoytova Anastasiya S. Rabokon Anastasiia M. popup.nrat_date 2020-04-02 Close