Search academic texts

Information × Registration Number 0219U102308, 0119U103471 , R & D reports Title Development of the biosafety criteria for adipose-derived stem cells popup.stage_title Head Tsupykov Oleg M., Кандидат медичних наук Registration Date 26-12-2019 Organization Bogomolets Institute of Physiology popup.description2  A key aspect in regenerative medicine is the adequate selection of cell transplants to ensure their biological safety and effectiveness. In an in vivo model of ovarioectomy in young and old CBA/Ca mice abnormalities in ovarian endocrine function were reproduced. Multipotent mesenchymal stromal cells (MMSCs) were obtained from adipose tissue, phenotyped by flow cytometry, and the in vitro characteristics of morphology, proliferative potential and ability to direct multilinear differentiation in osteogenic and adipogenic directions were evaluated. It was shown that under the influence of ovariectomy in young animals there is a decrease in proliferative potential, as well as an increase in adipogenic and a decrease in osteogenic potential of MMSCs. A decrease in colony-forming ability in young mice after ovariectomy was first shown. The results of the study indicate a possible effect of menopause on the functional activity of MMSCs. Product Description popup.authors popup.nrat_date 2020-04-02 Close