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Information × Registration Number 0220U000523, 0119U102610 , R & D reports Title Research of genetic and physiological fetures of drought-resistant transgenic wheat plants, obtaiened using siRNA technology popup.stage_title Head Kulesh Svitlana Sergiivna, Registration Date 25-02-2020 Organization Institute of Plant Physiology and Genetics NASU popup.description2 Transgenic plants of soft wheat variety Zymojarka were obtained Agrobacterium-mediated transformation with a double-stranded RNA suppressor of the prolinedehydrogenase (pdh) gene. The transgenicity of the obtained plants was confirmed by PCR analysis. T1-T2 generation was obtained from seed generation T0.Transformants have been shown to grow on selective mannitol media faster control. Plants of the seed generation T2 were characterized by decreased activity of the prolinedehydrogenase which is manifested in the changed of norm-stress-norm conditions.Transformants to have higher levels of free L-proline accumulation, higher resistance to water deficiency, and yields. Cytogenetic analysis revealed a significantly higher level of karyotypic variability of transgenic genotypes than non-transgenic ones. The chromosomal variability of transformants was manifested by aneuploidy. Product Description popup.authors Зборівська О.В. Сандецька Н.В. Шегеда І.М. popup.nrat_date 2020-07-03 Close