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Information × Registration Number 0220U001167, 0115U001972 , R & D reports Title The generation of novel bioactive osteoplastic materials containig bone morphogenetic proteins for osteogenesis induction. popup.stage_title Head Korchynskyi Olexandr, Registration Date 30-01-2020 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 As a result of the work on the project we generated the highly efficient biodegradable osteoplastic materials with incorporation of bone morphogenetic proteins for induction of osteogenesis. We have performed in vitro comparison and optimization of ratios for different bone morphogenetic proteins (BMP) while aiming to improve their osteoinductive properties. We have found that BMP preparations generated as a result of cotransfection of 85% of optimized BMP2 and 15% BMP7 plasmids are most efficient inducers of early osteogenesis of mesenchymal stem cells (MSC). We also found that Secreted Phosphoprotein 24, Spp24 is a promicing modulator of BMP osteogenic effects and elucidated a molecular mechanism mediating a positive cooperation of BMP and SPP24 effects in osteogenesis. Polyuretan osteoplastic materials were generated as a cooperative effort with Gdansk Polytechnical Univarsity, Gdansk, Poland. We performed the optimization in vitro of conditions for incorporation of mesenchymal precursor cells into polyuretan osteoinductive matrices with incorporated BMP. Such data will be an important contribution to both deeper understanding of basic molecular mechanisms in BMP signalling and practical use in translational biomedicine and medical practice.5481 Product Description popup.authors Луцик Максим Дмитрович Стойка Ростислав Степанович popup.nrat_date 2020-04-02 Close