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Information × Registration Number 0220U101386, 0118U005174 , R & D reports Title Development of "smart" portable signaling device for general toxicity of solutions based on enzyme multibiosensor. Section 2. Creation of bioselective elements of sensors and protocols for the determination of toxic substances popup.stage_title Head Soldatkin Oleksandr O., Доктор біологічних наук Registration Date 10-02-2020 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  In the course of the work, the prospects of several variants of coimmobilization of enzymes (urease, butylcholinesterase, glucose oxidase) with particles of different zeolites (crosslinking with glutaraldehyde in BSA membrane, physical adsorption, etc.) were tested. A number of potentiometric monobiosensors were developed based on the coimmobilization of enzymes with different types of zeolite on a pH-sensitive FET, and their performance was determined and compared. Clinoptilolite and Beta zeolite (BEA) have been shown to be more promising for biosensors than zeolite A. By changing the concentration of BEA zeolite in membranes, it is possible to extend the linear measurement range of the biosensor. It is known that a two-layer method of applying an enzyme with clinoptilolite provides a significant increase in the sensitivity of biosensors to substrates, whereas thermal modification of BEA zeolite crystals can improve the analytical characteristics of biosensors. The optimum concentration of zeolites in bioselective elements, at which the highest sensitivity to the biosensor was observed, was 10%. The ratio of Si / Al during synthesis and the diameter of the obtained particles affects the characteristics of the biosensor. The results show that it is possible to adjust the characteristics of enzyme biosensors by modifying the bioselective elements with zeolites. Product Description popup.authors Arkhypova Valentyna M. Bakhmachuk Anna O. Dziadevych Sergii V. Kukla Oleksandr L. Rachkov Oleksandr E. Saiapina Olha Ya. Shkotova Liudmyla V. popup.nrat_date 2020-04-02 Close