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Information × Registration Number 0220U103638, 0115U004187 , R & D reports Title Using molecular and cellular technologies for biotech wheat and maize plants resistant to the herbicide glyphosate obtaining popup.stage_title Head Morhun Bohdan V, Registration Date 29-10-2020 Organization Institute of Cell Biology and Genetic Engineering popup.description2 The purpose of the work was to obtain biotechnological plants of wheat and corn resistant to the herbicide glyphosate by genetic engineering, to improve the methods of Agrobacterium-mediated transformation in vitro of wheat and corn using molecular and cellular technologies, to obtain transgenic plants of wheat, to propagate them in vitro and to introduce them into the soil under a closed system. The following set of tasks was performed: Agrobacterium-mediated transformation of 18-day callus obtained from apical meristems of Podolyanka and Yatran 60 cultivars (621 pieces - Podolyanka and 638 pieces - Yatran 60 ) was performed, with a genetic vector whose T-DNA contains genes CP4 epsps (glyphosate resistance) and nptII (kanamycin resistance), according to the results of PCR analysis of 4 regenerating plants of Podolyanka and 5 plants of Yatran 60. One regenerating wheat plant of Podolyanka only turned out to be transgenic while all plants of Yatran 60 turned out to be transgenic. The selective concentration of kanamycin and paromomycin for callus crops of wheat cultivars Podolyanka, Zimoyarka and Yatran-60 was established: for Yatran 60 - 125 mg/l kanamycin and 150 mg/l paromomycin, for the cultivar Zimoyarka - 50 mg/l kanamycin and 150 mg/l paromomycin, for Podolyanka -25 mg/l kanamycin and 125 mg/l paromomycin. Paromomycin had a slight inhibitory effect on the morphogenetic potential of corn callus. Agrobacterium-mediated transformation of corn callus R2PLS61×PLS61 was carried out with vectors pCB135 and pCB133. PCR results obtained 4 and 1 regenerant, respectively, which contained the transgene CP4 epsps. PCR analysis of DNA of greenhouse plants obtained after Agrobacterium-mediated transformation of immature maize embryos did not reveal transgenes in plant DNA. The influence of vector regulatory elements on the degree of expression of heterologous genes in cereals has been clarified. Product Description popup.authors Bannikova Maria О. Morgun Bogdan V. Nitovska Iryna O. popup.nrat_date 2021-03-17 Close