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0220U104178,
0116U003092
,
To develop the effective technology of creation of homozygous material of cabbage cultures by the microspore culture
Soroka Anatolii I, Доктор сільськогосподарських наук
14-12-2020
Institute of Oilseed Crops of the National Academy of Agrarian Sciences of Ukraine
The scientific novelty of the work is that for the first time on the valuable breeding material of rapeseed it was found that the use of post-treatment of microspores at 35-37°C for one day instead of 32°C and simultaneous addition to the nutrient medium of phytohormones of two types (cytokinin and auxin) instead of one (cytokinin) at the concentration of 0.25 mg/l, as well as the replacement of the phytohormone 6-benzylaminopurine (BAP) with thidiazuron (TDZ), contribute to the better formation of haploid structures in the microspore culture of rapeseed. The obtained results: An effective technology for obtaining doubled haploids of rapeseed through microspore culture, which is reflected in the methodological recommendations, has been developed. For obtaining the maximum number of haploid structures in the culture of rapeseed microspores we can propose such optimal elements of the technology as: pre-treatment of inflorescences or buds at low temperature of 6-7°C for 2 days, microspore culture in a liquid nutrient medium without thickener, post-treatment of microspores at the temperature of 35-37°C, simultaneous addition to the nutrient medium of phytohormones of two types (cytokinin and auxin), or replacement of phytohormone BAP with TDZ. The practical value of the results is that the developed technology will allow to obtain doubled haploids on the valuable breeding material of rapeseed with a higher frequency in in vitro culture
Zinchenko Oleksiy S
Tigova Anna V
2020-12-14
Updated: 2025-12-08
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