Search academic texts

Registration Number

0221U000179, 0120U000107 , R & D reports

Title

Development of arginine-selective sensors for the analysis of food and dietary supplements used in preventive and rehabilitation medicine

popup.stage_title

Head

Saiapina Olha Ya,

Registration Date

03-01-2021

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

popup.description2

The main aim of the project is to develop highly sensitive and selective electrochemical bio- and chemosensors for determination of arginine and to optimize the composition of their (bio)selective elements and parameters of the working solutions for the further application in the analysis of food and dietary supplements used in the diet of therapeutic and prophylactic and rehabilitation purposes. As a result of the first stage of research, new electrochemical (bio)sensors for determination of arginine in solutions were developed. In particular, laboratory prototypes of conductometric biosensors based on arginase, urease and ammonium-selective zeolite clinoptilolite have been developed. The optimal ratios and conditions of immobilization of enzymes and zeolite in the selective elements of biosensors are determined. The influence of the method of co-immobilization of enzymes and clinoptilolite on the main analytical characteristics of the developed biosensors was investigated. The developed biosensors were compared with an arginine biosensor, which was not modified with clinoptilolite, in such parameters as sensitivity, linear and dynamic ranges, limit of detection, response time, operational and storage stability. Calibration curves for determination of arginine using the developed biosensors in buffer solutions of different composition were obtained. The laboratory prototypes of conductometric chemosensors based on arginine-sensitive derivatives of calixarenes were developed. The optimal concentration of calixarene-methylenebisphosphonic acid in the selective element of chemosensor was determined and the working characteristics of the corresponding chemosensors (sensitivity, limit of detection, linear range, signal reproducibility) were analyzed. Selectivity of the developed chemosensors was studied. The influence of the buffer solution parameters on operation of the chemosensor based on calixarene-phosphinic acid and its analytical characteristics were studied.

Product Description

popup.authors

Kucherenko Daria Yu.
Nesterenko Yevheniya О.
Saiapina Olha Ya.
Soldatkin Oleksandr O.
Yarynka Dariia V.

popup.nrat_date

2021-01-03