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Information × Registration Number 0221U101315, 0120U101972 , R & D reports Title Composite nerve conduits on the basis of polymer nanofibrils and electro-conducting materials for treatment of critical neuronal injuries popup.stage_title Head Kyrylenko Sergiy D., Кандидат біологічних наук Registration Date 19-01-2021 Organization Sumy State University popup.description2  The object of research: specific features of the process of hemostasis using a new hemostatic material manufactured by electrospinning out of the natural polymer (chitosan). The subject of research: to study biocompatibility and hemostatic efficiency of chitosan membranes manufactured by electrospinning, in comparison with the ordinary chitosan sponge and chitosan aerogel on the basis of evaluation of their functional properties. The goals of the research: to study the porosity of materials based on chitosan depending on the method of their manufacture; to study the dynamics of degradation (biodegradation) of chitosan materials; to analyze the viability of cells on the basis of indicators of their adhesion and proliferation at different times of the study; to compare the antibacterial action of different materials based on chitosan against E. coli and S. aureus in the dynamics of the experiment; to test in vitro the interaction of materials with blood components and evaluate the critical parameters of activation of the hemostasis system; to investigate the hemostatic and bioactive efficacy of the materials in vivo using a model of hepatic bleeding in rats; to evaluate tissue samples histologically to assess the degradation of biomaterials, the hosts immune response and the degree of liver regeneration. Research methods used: determination of porosity of materials using scanning electron microscopy; studying of degradation and biodegradation in vitro; experiment on cell cultures; bacteriological research; studying of hemostasis in vitro; assessment of biodegradation and hemostatic properties of drugs in vivo; histological and immunohistochemical studies. Practical outcomes: Highly porous biocompatible electrospinning membranes capable of biodegradation and hemostatic interaction with blood in vitro have been developed. Product Description popup.authors Bezkorovaina Nadiia I Kazban Viktoriia S Korniienko Viktoriya V. Liubchak Iryna V. Yanovska Hanna O popup.nrat_date 2021-01-19 Close