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Registration Number

0221U103005, 0116U001167 , R & D reports

Title

Molecular genetic polimorphism of wine yeast Saccharomyces cerevisiaecharacterisation as a component of their selection and wine technologies

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Head

Muljukina Nina Anatoliyivna, Доктор сільськогосподарських наук

Registration Date

12-02-2021

Organization

National Scientific Centre "V.Ye. Tairov Institute of Viticulture and Wine-making"

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70 autochthonous wine yeast samples Saccharomyces cerevisiae isolated in vineyards in different regions of Ukraine from different varieties were deposited in the 3 largest collections of microbial cultures in the world, located in Japan, USA and Great Britain. Selected samples are characterized by a variety of sources of isolation (wine ancient varieties, wine varieties breeded at NSC “Tairov Research Institute of Viticulture and Wine-Making” and by different plots and regions (Odessa (Ovidiopol and Bolgrad districts), Mykolayiv and Kherson regions), which is the basis of their spatial and geographical diversity. In the Gene Bank of Japan DNA identification of Saccharomyces cerevisiae strains by loci - ITS1_5.8S_ITS2 (ribosomal RNA genes) was carried out. The heterogeneity of strains in biochemical and molecular genetic terms is shown. Among the biochemical traits, the activity of enzymes that catalyze the reactions of plastic and energy metabolism, as well as the content of organic acids was studied. Differences in the level of enzymes activity were found. Preliminary evaluation of the results obtained in the study of wine yeast strains USRCBY-3645 - 3649 oon wine quality demonstrate the practical prospects of this work. To differentiate the strains of wine yeast and assess their phylogenetic relationships sequences obtained from the Gene Bank of Japan were used. Isolated DNA of 43 strains and allelic characteristics of microsatellite loci were used to differentiate wine yeast strains, too and the study of their phylogenetic relationships with strains of other geographical origin were done. Visualization of he analysis results allowed to draw a conclusion about the clustering of wine yeast samples from Ukraine with ones of European wine-growing countries. Other samples formed 3 separate subclusters, relatively far from the reference samples.

Product Description

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Karastan Olga M
Papina Olena S

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2021-02-12