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Information × Registration Number 0221U103159, 0118U000201 , R & D reports Title Identification (search) of a new antimicrobial compounds' producers against multi-resistant pathogenic microbes from the Black Sea microbiota. popup.stage_title Head Ivanitsya Volodymyr O., Доктор біологічних наук Registration Date 15-02-2021 Organization Odessa I. I. Mechnikov National University popup.description2 Aerobic spore-forming bacteria were discovered in deep-water sediments of the Black Sea with extreme anaerobic conditions, their biological diversity was characterized, as well as biological properties, structure of genomes and exometabolomes of antagonistically active strains and their potential for biotechnological research. It was for the first time established that Black sea deep-sea bottom sediments are a source of strains that have unique metabolic characteristics. Screening was performed among isolates for the presence of antagonistic activity against a set of test bacteria, filamentous fungi and yeast. A collection of new unique strains producing antimicrobial compounds has been created and the spectrum of these compounds has been studied. Compounds of the classes of fengicins, surfactins and gageotetrins were found. For the first time was carried out the search and identification of genes and clusters of genes that encode biologically active compounds with antimicrobial activity, the selection of strains producing antibiotics. Were established the optimal conditions and composition of the environment for maximum yield of antimicrobial compounds. Producer genomes are sequenced and annotated. For the first time, a comparison of the spectra and potential of naturally preserved years spore-forming bacteria isolated from the deep-sea soil of the Black Sea and bacteria of this group from surface waters, soil and aquatic organisms regarding to antimicrobial metabolites. The strain of marine spore-forming bacteria Bacillus velezensis ONU553, which is a producer of lipopeptide antibiotics, has phytostimulatory and antagonistic properties against phytopathogenic fungi Fusarium oxysporum 54201, as well as opportunistic and bacteria Proteus vulgaris ATCC 6896, Staphylococcus aureus ATCC 6538 P, Escherichia coli ATCC 25922, Salmonella enterica NCTC 6017, Klebsiella pneumoniae ATCC 10031, can be recommended for the development of antibiotics and used as a basis for pro  Product Description popup.authors Ivanytsia Tetiana V Vasylieva Natalia Yu Gorshkova Olena H Gudzenko Tetiana V Sashchuk Olena V Strashnova Iryna V Tesliuk Natalia I Tytarenko Nadiia V Shtenikov Mykola D popup.nrat_date 2021-02-15 Close