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Information × Registration Number 0221U106654, 0120U104770 , R & D reports Title Development of fluorescent diagnostic materials for hybridization assays and studies by polymerase chain reaction popup.stage_title Head Tatarets Anatolii L., Registration Date 16-12-2021 Organization State Scientific Institution Scientific and Technological Complex" Institute of Single Crystals "of the National Academy of Sciences of Ukraine popup.description2 Purpose of work – development of domestic fluorescent materials for the hybridization analysis, the PCR diagnostics of genetic and infectious diseases, in particular virus of COVID 19, the detection of genetically modified organisms (GMOs) and other nucleic acid studies. Main tasks of the project: – Synthesis of monomeric and dimeric dyes-intercalators, as well as dyes with fluorescence lifetime (FLT) sensitive to the environment (FLT-sensitive); – Investigation of photophysical properties of the obtained dyes and evaluation of their suitability for the development of methods for DNA determination. – Development of PCR tests based on intercalating dyes and hybridization assay methods based on FLT-sensitive dyes. Research objects – monomeric and dimeric dyes-intercalators based on derivatives of acridine orange and monomethine cyanine dyes; FLT-sensitive fluorescent dyes of different classes. Research methods – organic synthesis, spectral and other physical and photophysical research methods – NMR-spectroscopy, mass-spectrometry, electron absorption and fluorescence spectroscopy. Results obtained during the project implementation: – Methods for the synthesis of new monomeric and dimeric intercalating dyes have been developed. Using this method ten new homodimeric intercalating dyes based on acridine orange have been obtained. New dyes are more photolytically, thermally and hydrolytically stable compared to commercially available cyanine dyes. – Developed and improved methods for obtaining structural analogues of commercial dyes-intercalators SYBR Green I and YOYO-1. – Synthesis of reactive FLT-sensitive dyes of various classes of fluorescent compounds as well as investigation of their spectral and photophysical properties were performed. Among obtained dyes the most suitable as labels were derivatives of squaraine dyes. Product Description popup.authors popup.nrat_date 2021-12-16 Close