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Information × Registration Number 0221U106745, 0120U104201 , R & D reports Title Development of original combinations of antihypertensive agents, their analysis and standardization. popup.stage_title Head Logoyda Liliya S., Доктор фармацевтичних наук Registration Date 18-12-2021 Organization I. Horbachevsky Ternopil State Medical University popup.description2  GDR report: 131 pages, 40 figures, 46 tables, 145 sources. ANTIHYPERTENSIVE MEDICINES, PHARMACEUTICAL ANALYSIS, VALIDATION, BIOEQUIVALENCE. Object of research - Antihypertensive drugs (valsartan, atorvastatin, lisinopril): substances and tablets of industrial production; monolayer of Saso-2 cells. The purpose of the study: development of analytical methods for determining the original combinations of antihypertensive drugs and standardization of API in substances and drugs. Research methods: For the development of analytical methods of analysis were used - UV and visible spectrophotometry, TLC, HPLC / MS / MS. Spectrophotometric methods for the determination of valsartan in substances and drugs by reaction with bromophenol blue (BFS) and methyl red (MC) have been developed and validated. Previous studies have shown that non-extractive binary complexes between valsartan and BFS and MCH are formed on the basis of ionic associates. Valsartan forms ion-pair complexes selectively with dyes, as evidenced by the formation of a colored complex with BFS at pH 5.5 with ? max at 424 nm and MCH at pH 4.3 with ? max at 494 nm. The formed ionic associates are sparingly soluble in water, but in optimized experimental conditions are freely soluble and do not require extraction with organic solvents, which is a significant advantage of the developed analytical methods. Optimization of the conditions for spectrophotometric determination is necessary for the rapid and quantitative formation of color complexes of ionic associates with maximum stability and sensitivity. Maximum absorption was observed in a solution of methanol with BFS, while ethanol, propanol, chloroform, acetonitrile and ethyl acetate were unsuitable. Maximum absorption was observed in ethanol solution with MC, while methanol, propanol, chloroform, acetonitrile and ethyl acetate were unsuitable. At the next stage the stability of the analyzed solution in time is investigated. Product Description popup.authors popup.nrat_date 2021-12-18 Close