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Information × Registration Number 0221U107126, 0120U104768 , R & D reports Title Plant-based synthesis of recombinant pharmaceutical proteins capable to prevent some infectious diseases of bacterial and viral origin. popup.stage_title Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 27-12-2021 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description2 For the first time, the transgenic tomato plants able to express the antiviral protein of human interferon (HuIFNα-2b) were obtained. Human interferon HuIFNα-2b in the transgenic tomato plants demonstrated the biological activity both for the in vitro model system (the virus-infected monkey cell culture) and in vivo in case of infecting the mice with vesicular stomatitis virus. Here the biological activity was hundred times higher comparing to the other transgenic plants and came up to 850*103 IU per 1 g FW in the transgenic tomato leaves. In the course of the project, for the first time, we produced the edible transgenic plants characterized by confirmed expression of the antibacterial protein-bacteriocin - colicin M. The antibacterial activity (demonstrated via the termination of growth rate the various Escherichia coli strains) was proved for the transgenic leafy (salad, mizuna, kale) and taproot (carrot) plants. Accumulation of the recombinant colicinin M in transgenic lettuce plants was about 50 μg per 1 mg FW, and the antibacterial activity of the plant extracts averaged in 4 * 103 IU. As the F1 generation of the transgenic annual plants of short term vegetation (lettuce, mizuna, tomatoes) was produced, the inheritance of the transgenes and the transgenic traits was confirmed. The presence of recombinant colicinin M in the transgenic plants was proved by Western blot analysis. The retention of the functional activity of both pharmaceutical proteins (interferon and colicin M) was confirmed for the dried plant material, which enables the usage of the dried transgenic plant powder as a pharmacy. Product Description popup.authors Kyriienko Anastasiia V. Lystvan Kateryna V. Luchakivska Yuliya S. Matvieieva Nadiia Anatoliivna Ovcharenko Olga O Rudas Volodymyr А. Symonenko Yurij V. Shcherbаk Nаtаliia L. Yaroshko Olga M popup.nrat_date 2022-03-09 Close