Search academic texts

Information × Registration Number 0222U000473, 0120U000182 , R & D reports Title Study of therapeutic effect of mesenchymal stem cells towards neuroinflammation-induced cognitive impairment. popup.stage_title Head Skok Maryna Volodymyrivna, Доктор біологічних наук Registration Date 10-01-2022 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description2 Chronic neuroinflammation causes cognitive impairment and accompanies age-dependent neurodegenerative disorders, like Parkinson and Alzheimer diseases. Nicotinic acetylcholine receptors (nAChRs) are exressed in the brain neurons, astrocytes and microglia and are involved in regulation of neuroiflammation, learning and memory. Mesenchimal stem cells (MSCs) produce multiple neurotrophic factors supporting neurogenesis and neuroregeneration. Previously we reported that MSCs obtained from the human umbilical cord and injected intravenously into mice are able to penetrate the brain and restore episodic memory, as well as the nAChR and amyloid-beta levels impaired upon neuroinflammation. As a result of the present project, we demonstrate that therapeutic effectof a single MSCs injection is stable during at least four months. In contrast, the effect of regular injections of MSC-conditioned medium is transient and is accompanied by the immune response to the medium components. The optimal MSC dose is 106 cells per mouse; the use of chemokine SDF1 allows decreasing the dose by an order. MSCs are able not only prevent neuroinflammation, but also cure its negative consequences after two months of regular LPS injections. MSCs of various origins (from either umbilical cord or adipose tissue) are equally important and can be introduced both intravenously and intraperitoneally. MSCs stimulate proliferation of neural precursor cells in the brain; SDF1 increases their proliferation and potentiates MSCs influence on the brain and memory. In whole, the data obtained demonstrated a significant MSCs potential for treating neuroinflammation-induced cognitive disorders.  Product Description popup.authors Izmailov Mykhailo Rustenovych Kalashnyk Olena Mykolaivna Koval Lyudmyla Mykolayivna Lykhmus Olena Yuriyivna popup.nrat_date 2022-03-09 Close