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Information × Registration Number 0222U000561, 0121U109268 , R & D reports Title Identification and elucidation of the role of new structural and regulatory genes in riboflavin oversynthesis in flavinogenic yeast popup.stage_title Head Sibirny Andrij Andrijovych, Registration Date 13-01-2022 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2  Species-specific regulation of C. famata SEF1 gene transcription was studied. The SEF1 gene promoters Candida albicans and Candida tropicalis have been shown to restore riboflavin oversynthesis in C. famata strain with deletion of the SEF1 gene. The non-flavinogenic yeast gene Scheffersomyces stipitis was cloned to obtain C. famata strains expressing this gene, which will be used to elucidate the role of SEF1 in riboflavin supersynthesis in yeast. C. famata yeast strains were constructed with an additional copy of the central regulatory gene SEF1 under the control of the promoter of the LAC4 gene (encoding β-galactosidase) gene C. famata based on existing riboflavin producers. The effect of SEF1 gene expression under the control of these promoters on riboflavin production under different growing conditions was studied. Recombinant strains of C. famata containing the expression cassette “pLAC4_cf-SEF1_cf” were found to have 1.6–2.1 times higher yields of riboflavin in lactose as the only source of carbon compared to the parent strain AF4. Production of riboflavin by engineered strains when grown in flasks reached 1.7 g / l. The increase in riboflavin synthesis due to overexpression of the SEF1 gene under the control of the TEF1 promoter was insignificant. The engineered strains of C. famata, which contain an additional copy of the SEF1 gene under the control of the lactose-inducible promoter LAC4, are an ideal platform for the development of industrial production of riboflavin in a by-product of the dairy industry - whey. The obtained results allowed to identify new promising targets for metabolic engineering of flavinogenic strains of C. famata in order to improve the production of vitamin B2. Product Description popup.authors Andreeva Yulia Andrijivna Bandura Yurij Oleksandrovych Dzanajeva Lubov S. Dmytruk Kostiantyn Vasylovych Petrovska Yana Oleksandrivna. Fayura Lubov Romanivna Fedorovych Dariya Vasylivna Tsaruk Aksynia Yurijivna Tsyrulnyk Andriy O. popup.nrat_date 2022-03-09 Close