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Information × Registration Number 0222U000574, 0119U100697 , R & D reports Title Study of the role of transcriptional and translational changes of the ATM / CHEK2 / P53 / PUMA molecular pathway in the formation carcinogenic effects in the immune system of Chernobyl clean-up workers in the remote period after exposure. popup.stage_title Head Bazyka Dimitry A., Доктор медичних наук Registration Date 13-01-2022 Organization State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine" popup.description2 Report: 183 pages, 35 figures, tables 36, 1 formula, 3 appendices, 210 sources. GENE EXPRESSION, IMMUNE SYSTEM, IONIZING RADIATION, CELL CYCLE, GENOME INSTABILITY. Object of the study are peripheral blood lymphocytes of 200 Chernobyl clean-up workers, 40 patients from the positive control group and non-exposed control group from 60 subjects. Aim. To determine the pathogenetic significance and dose dependence of ATM/CHEK2/P53/PUMA cascade gene expression in combination with biological markers of ionizing radiation to quantify radiation-induced effects in the immune system. Methods - immunological, molecular-genetic, cultural, statistical, analytical, bioinformatical analysis. Results. Molecular genetic markers of ionizing radiation in the formation of late cancer effects of radiation in Chernobyl clean-up workers were determined: the increased levels of expression of histone γ-H2AX, spontaneous level of Cyclin D1, relative telomere length, violation of Cyclin D1-dependent regulation of the cell cycle of peripheral blood lymphocytes and imbalance of T/B-cells immunity. Activation of gene regulation of reparation, apoptosis, cell cycle and aging in Chernobyl clean-up workers and patients with malignant neoplasms of the oral cavity, oral and laryngeal part of the pharynx were determined. Common dominant molecular genetic pathways with the same direction were determined for all data sets of ATM/CHEK2/P53/PUMA gene expression cascade for the examined groups of Chernobyl clean-up workers and patients with malignant neoplasms, which are associated with gene regulation of DNA repair; TP53-mediated regulation of transcription of cell death genes, transcription of genes involved in the release of cytochrome C and transcription of DNA repair genes; NOTCH signaling and pre-NOTCH expression and processing. Implementation: 22 publications, 27 reports, 1 innovative technology. Field of application - radiation medicine, molecular biology, immunology. Product Description popup.authors Ilienko Iryna Mykolaivna Belayev Oleg Anatoliyovich Bazyka Dimitry Anatoliyovich Vorobyov Mykola Olegovich Golyarnik Nataliya Anatoliivna Zvarych Liliia Mikolaivna Lyaskivska Olena Viktorivna popup.nrat_date 2022-03-09 Close