Search academic texts

Information × Registration Number 0222U002730, 0117U000852 , R & D reports Title Study of molecular mechanisms of tolerogenic activity of cryopreserved dendritic cells in their adoptive application. popup.stage_title Head Dubrava Tetiana Н., Кандидат біологічних наук Registration Date 15-02-2022 Organization Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description2  Research object was bone marrow mononuclear and dendiritc cells of CBA/H mice weighing 20-24g. Research aim was to investigate the peculiarities of ultralow temperatures effect on molecular mechanisms of implementing the tolerogenic activity of dendritic cells (DCs) for optimization of cryopreservation and further establishing the banks for their preservation and adoptive usage in clinical practice when treating autoimmune diseases. Research methods were as follows: assessment of structural and functional characteristics of mononuclears and dendritic cells after cryopreservation by means of culturing in vitro, inverted and light microscopy, flow cytometry and real time PCR. Owing to theoretical grounding the method for cryopreservation of bone marrow MNCs under 10% DMSO protection with the rate of 1 deg/ min down to -40ºC with following plunging into liquid nitrogen, which provided structural and functional integrity of the obtained from them ex vivo immature DC with activation of heat shock proteins hsp70 in them and expression of GILS gene, tolerogenic activity regulators. It has been established that the DCs obtained in culture with cryopreserved on the selected regimen MNCS had a maximum ability to elevated content and suppressor activity of T-regulatory cells with foxp3 gene activation and stimulation of the production of anti-inflammatory cytokine IL-10. This resulted in maximal decrease of cell manifestation of adjuvant arthritis in animals, that explained molecular mechanisms of tolerogenic activity of cryopreserved dendritic cells during their adoptive application. Product Description popup.authors Babenko Natalya M. Batsunova Lyudmyla V. Bondarovych Mykola O Gaevska Yliia O. Goltsev Anatoliy M. Zubov Pavlo Mykhajlovych Lutsenko Olena D Nardid Oleh A. Ostankov Maksym V. Ostankova Lyudmyla V. Pushkova Evgenia M. Yampolska Kateryna Ye. popup.nrat_date 2022-03-09 Close