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Information × Registration Number 0222U005180, 0122U002240 , R & D reports Title The role of immunomodulatory in the antitumor action of traditional and experimental medicines at their delivery by polymeric nanocarriers to various target cells in vitro and in vivo popup.stage_title Head Stoika Rostyslav S., д.б.н. Registration Date 24-12-2022 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2 As part of the 1st stage of the work, thiazolidinone and 1,2,3-triazole derivatives were selected for their ability to interact with DNA, thiols, and other model nucleophiles, and the effect of these substances on cells with different levels of expression of DNA damage repair enzymes and with different content of glutathione. The obtained results make it possible to explain the mechanisms of structural and functional changes in target cells under the action of the studied heterocyclic compounds. As part of the 2nd stage of the work, the ability of traditional (doxorubicin, oxaliplatin) and experimental anticancer drugs (4 different thiosemicarbazone derivatives - triapine ((3-AP), COTI-2, DpC, KP-2550) to induce immunogenic cell death in a mouse melanoma model was compared B16, and to prevent the development of this tumor in mice of the C57BL/6 line upon administration of a cell vaccine from B16F10/wt melanoma cells treated with the indicated drugs. Derivatives of thiosemicarbazones with the highest ability to induce immunogenic cell death have been found. As part of the 3rd stage of the work, a comparative study of the effect of the complex of doxorubicin with a polymer carrier modified with a cannabimimetic NSE, and native doxorubicin (Dx) and a free polymer that does not contain NSE, on the death of tumor and pseudonormal cells. The pro-apoptotic effect of NSE in vitro experiments and the bioprotective effect of NSE in vivo experiments were established. As part of the 4th stage of work, chitosan preparations with a molecular weight of 30-50 kDa and 5-10 kDa and improved solubility in an aqueous medium with physiological pH values were purified. On the basis of these preparations, composites with antibiotics, which have a more toxic effect on microorganisms, as well as composites with antitumor factors, which have a more prolonged effect in the body of laboratory animals and are not toxic to isolated human blood lymphocytes, have been created. Product Description popup.authors Ivasechko Iryna Ihorivna Antoniuk Volodymyr Oleksandrovych Kashchak Nataliya Ivanivna Klishch Mykola Vasylovych Klyuchivska Olga Yuriivna Kozak Yuliia S. Lootsik Maxim Dmytrovych Manko Nazar Olegovych Panchuk Rostyslav R. Senkiv Yuliya V. Skorokhyd Nadiya Romanivna Starykovych Marina O. Stoika Rostyslav Stepanovych Finiuk Nataliya Stepanivna popup.nrat_date 2022-12-24 Close