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Information × Registration Number 0223U000002, 0120U100747 , R & D reports Title Study of molecular-genetic features of pathogenesis of Ph-negative myeloproliferative neoplasia in persons who have been exposed to ionizing radiation due to the Chernobyl accident popup.stage_title Head Neumerzhytska Liubov V., Кандидат біологічних наук Registration Date 01-01-2023 Organization State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine" popup.description2  The object of the study is the genomic DNA of 330 patients with Rh-negative myeloproliferative neoplasia (MPN), a database of 4737 medical history records of patients with MPN.The purpose of the work is to determine the molecular and genetic features of the pathogenesis of Rh-negative myeloproliferative neoplasias in people exposed to ionizing radiation as a result of the accident at the Chernobyl nuclear power plant, and to improve the effectiveness of their diagnosis.Research methods are molecular genetic (PCR), analytical, statistical.It was established that patients with a history of radiation more often have a negative status for JAK2 mutation than those who fell ill spontaneously: (24.41 ± 1.69) % versus (37.82 ± 7.66) %, p < 0.001). With JAK2 positive mutational status, men predominate in both groups. An increase in thrombotic complications was found in the group of patients with a history of radiation: (18.30 ± 1.44) % versus (9.38 ± 0.46) %, (p < 0.001). No correlation was found between the presence of a JAK2 mutation and the frequency of thrombotic events in patients in each group: with a positive mutational status, thrombosis in the 1st group accounted for (13.06 ± 2.53)%, with a negative - (20.0 ± 1.70) %, in the 2nd group, respectively (5.60 ± 0.59) % and (11.69 ± 0.64) %. Thrombocytosis in men is directly correlated with age, in women there is a bimodal distribution by age. It was established that JAK2 gene mutation is not the only key molecular genetic marker in the prognosis of thrombotic complications. The presence of additional non-specific mutations in genes of epigenetic regulation, which was detected in patients with a history of radiation, probably precedes the development of MPN with a high risk of thrombotic complications (with the presence of mutations - (55.5 ± 16.5) %, without mutations - (15, 4 ± 10.1) %) and can testify to the peculiarities of radiation pathogenesis and the development of the disease of clonal hematopoiesis of uncert. Product Description popup.authors Verbylenko Roksana Mykolayivna Klymenko Serhii Viktorovych Neumerzhytska Liubov V. popup.nrat_date 2023-01-01 Close