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Information × Registration Number 0223U000606, 0122U002425 , R & D reports Title Identification of the Brassica rapa tubulin gene family and characterization of their genomic organization in different subspecies popup.stage_title Head Pirko Yaroslav V., Доктор біологічних наук Registration Date 12-01-2023 Organization Department for special training National academy of science of Ukraine at Kiev University popup.description2   During the first work stage a complete identification and characterization of the tubulin family genes in the rape (Brassica rapa) were carried out. Its exon-intron structure was determined, its belonging to the classes and isotypes of these proteins was established, its phylogenetic position in relation to other species tubulins was clarified, and the gene position on the ancestral Cruciferous karyotype genomic blocks was reconstructed. An analysis of the different species genomes synteny was carried out, which made it possible to identify the homologous relationship type of the identified genes and to clarify its evolutionary history. A total of 9 α-tubulin genes were found, 1 of which was an unexpressed pseudogene. Class I α-tubulins had 4 exons, and class II α-tubulins had 5 exons. 21 β-tubulin genes were identified, 5 of which were class III pseudogenes. It was found for the first time in dicotyledonous plants that in the case of B. rapa the conservatism of the exon-intron structure of β-tubulin is violated: the first intron is missing in the BraA.TUB2.b gene. Only one γ-tubulin gene has been identified in the B. rapa genome, BraA.TUG1.a., assigned to the 1st isotype. The γ-tubulin gene had 9 exons in its structure. The study of intragenomic synteny confirmed that copies of the BraA.TUA2, BraA.TUA5, BraA.TUB4, BraA.TUB7, and BraA.TUB9 genes arose as a result of genome-wide triplication. These onologues were not eliminated during the selection process, in contrast to the genes of other tubulin protein isotypes. For the conservative TUA2, TUA5, and TUB2 isotypes, it was possible to detect orthologs in the genomes of A. thaliana and A. lyrata only due to the reconstruction of the ACK block positions of in different species. The obtained data will be used for correct interpretation of the analysis data obtained at the next stage of the rape samples molecular genetic study, where will be used a marker system based on the assessment of tubulin gene intron length Product Description popup.authors Afanasyeva Kateryna S. Blume Rostislav Ya. Pirko Yaroslav Vasylyovuch Rabokon Anastasiya M. Saharowa Wladislava G. Sviridova Katerina O. Chopei Mariana Ihorivna popup.nrat_date 2023-01-12 Close