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Information × Registration Number 0223U000614, 0121U107989 , R & D reports Title Theoretical substantiation of creation of preparations on the basis of bacteriophages of causative agents of mastitis of cows popup.stage_title Head Kukhtyn Mykola D., д.вет.н. Registration Date 12-01-2023 Organization Ternopil Research Station Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine popup.description2  It was established that the most effective method for cleaning staphylococcal bacteriophages from bacterial cells is the use of multi-stage filtration, compared to heat treatment and the use of chloroform. All studied phages Phage SAvB07, Phage SAvB08, Phage SAvB12 and Phage SAvB14 were found to be sensitive to changes in the pH of the environment. The maximum replication of phages occurs at pH values рН of 6–7 units. It was found that among the bacteriophages we studied, the best lytic effect against cultures of S. aureus var. bovis exhibits Phage SAvB14 with a short latent period. The results of thermal stability studies showed that the phage SAvB14 bacteriophage partially loses its activity in the temperature range of 45–65 °C, which is an important factor in the technological features of phage drug production. The most optimal temperature for storing Phage SAvB14 is 4–8 °С. Phage SAvB14 is more resistant to the effects of high temperatures (45–65 ºС) and pH fluctuations, compared to other investigated phages, and therefore is better for creating a drug against staphylococcal mastitis of cows. Product Description popup.authors Boltyk Natalіa Petrіvna Vasylkiv Oleg Klymyk Vira T Kryzhanivskyi Yaroslav Yo Perkiy Yuriy Bogdanovich Rushchynska Tetiana popup.nrat_date 2023-01-12 Close