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Information × Registration Number 0223U001328, 0121U110167 , R & D reports Title Scientific basis for bioconversion of lignocellulosic agricultural wastes to second-generation ethanol using yeasts and mycelial fungi popup.stage_title Head Pidhorskyi Valentyn S., Registration Date 27-01-2023 Organization Institute of Microbiology and Virology. D.K. Zabolotny National Academy of Sciences of Ukraine popup.description2  Report: 39 pp., 11 tables, 4 figures, 32 sources. SCIENTIFIC BASIS OF THE BIOCONVERSION OF LIGNOCELLOSULIC AGRICULTURAL WASTE TO SECOND-GENERATION ETHANOL USING YEAST AND MYCELIAL FUNGI at the third stage. The purpose of the work is to optimize the conditions of plant biomass processing, the process of its enzymatic hydrolysis and saccharification using micromycete enzymes, and fermentation of the obtained sugar mixture with xylose-fermenting yeast to increase the efficiency of the bioethanol production process. Research methods - modern microbiological, biochemical methods, methods of mathematical statistics. As a result of the use of Plackett-Berman and Box-Behnken methods of mathematical statistics, the optimal conditions for the hydrolysis of wheat straw by the Penicillium funiculosum enzyme complex were established, namely, the concentration of alkali 4.6-4.8%, the power of microwave irradiation 1200W, pH 4, temperature 40°С , the duration of the process is 18 hours and the dilution of the culture liquid with a buffer solution 1:1. Under such optimized conditions, hydrolyzates of wheat straw contained from 5 to 7.5 g/l of reducing sugars, of which 0.7-1.0 g/l of glucose, 2.2-2.9 g/l of xylose and 0.7- 0.8 g/l cellobiose. Molecular genetic identification confirmed that the strain of xylose-fermenting yeast UKM Y-2810 isolated by us belongs to the species Scheffersomyces stipitis. Nucleotide sequences of ITS fragments (OP931914) and 28S rDNA gene (OP931915) of this strain were entered into the Genbank. Ethanologenic potential of strain Sc. stipitis UKM Y-2810 was studied according to the Box-Behnken plan, using actual concentrations of monosugars in syrups obtained by us during the optimized saccharification of lignocellulosic substrates with P. funiculosum enzymes. Under the conditions of static microaerophilic cultivation of strain Sc. stipitis UKM Y-2810 on model media with mixtures of glucose and xylose showed the highest rates of bioethanol production, whic Product Description popup.authors Hetman Katerina I. Dudka Halyna P. Yovenko Olga V. Kurchenko Iryna M. Nakonechna Lydia T. Pavlychenko Anna K. Syrchin Sergey O. Filipishena Oksana Y. Fomina Maryna O. Yurieva Olena M. Yanieva Olha D. popup.nrat_date 2023-01-27 Close