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Information × Registration Number 0223U002028, 0122U002204 , R & D reports Title Development of fluorescently tagged iron (II) clathrochelates, the study of their chemical properties, interaction with proteins, and biological activity in cancer cells popup.stage_title Head Chernii Svitlana V., к.б.н. Registration Date 07-02-2023 Organization Institute of General and Inorganic Chemistry. VI Vernadsky National Academy of Sciences of Ukraine popup.description2 Symmetric (9,10) and asymmetric (11,12) iron(II) tris-dioximate clathrochelate complexes with phenanthrene rib substituents, which have their fluorescence, were obtained. It was shown that symmetric phenanthrene clathrochelates do not bind to proteins, while asymmetric complexes show interaction. Furthermore, it was established that the intensity of protein fluorescence quenching depends on the number of phenanthrene substituents. Spectral studies showed the formation of BSA/11 and BSA/12 complexes. The ways of synthesis of new fluorophores containing in their structure a carboxyl fragment suitable for further bioconjugation are presented. It was established that the studied indolenine fluorophores localize in mitochondria (Pearson coefficient R = 0.31±0.09). Therefore, the most suitable fluorophore for further conjugation was selected among the commercially available fluorescent labels. Symmetric cationic trimethycyanine dyes with β-substituents in the polymethine chain have also been proposed as probes for nucleic acid detection and cell imaging. Spectral methods showed the interaction of all dyes with nucleic acids but by different binding mechanisms. Cytotoxicity of symmetric (9 and 10) and asymmetric (11 and 12) iron(II) tris-dioximate clathrochelate complexes against A2780 cells was determined by the MTT assay. It turned out that the cytotoxicity values are similar for all investigated metal complexes – about 60% at a concentration of 1 µM/l within 24 hours. Therefore, the introduction of different numbers of phenanthrene substituents does not affect the biological activity of the iron(II) clathrochelate complex. The project performers sincerely thank the employee of Friedrich-Alexander University, prof. Andrii Mohir for scientific advice, technical support (Germany), and the Institute of Science employees, prof. Ricardo Henriques for technical support and Ph.D. student D. Aristova for fluorescence microscopy (Portugal). Product Description popup.authors Denysenko Iryna M Mikhalyova Olena Selin Roman O. popup.nrat_date 2023-02-07 Close