Search academic texts

Information × Registration Number 0223U002504, 0118U000214 , R & D reports Title Functional, biological and physico-chemical properties of membrane and extracellular microorganisms macromolecules and their dependence from primary structure peculiarities popup.stage_title Head Varbanets Liudmyla D, д.б.н. Registration Date 22-02-2023 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description2  Isolated and characterized lipopolysaccharides (LPS) of representatives of Enterobacteriaceae: P. agglomerans. Studies of lipopolysaccharides (LPS) of P. agglomerans strains isolated from different host plants and obtained from different collections continued. The LPS studied were heterogeneous in terms of both monosaccharide and fatty acid composition. The structures of lipid A of four strains of P. agglomerans 8488, 7460, P1a, P324, which were represented by hexa-, penta-, tetra-, and triacyl forms, were established. The structures of their O-specific polysaccharides (OPS) were represented by linear di-, tri-, and tetrasaccharides (P324, P1A, and 8488, respectively), as well as a branched tetrasaccharide (7460). All structures are unique and established for the first time. It is shown that even slight differences in the structures of OPS, such as α- or monosaccharide configuration, affect the cross-reactivity of the studied lipopolysaccharides (LPS). The investigated LPS were characterized by different toxicity and pyrogenicity, which vary depending on the strain. Some of them were more pyrogenic than pharmaceutical pyrogenic, but none of the LPS tested was more toxic than E. coli O5:B55 LPS. All studied LPS are represented by a heterogeneous population, which includes two main types of molecules: high-molecular S-LPS with O-chains of different lengths and low-molecular R-LPS, which does not contain OPS chains. That is, the bimodal distribution typical for S-forms of LPS is shown. Many active producers of mannanases, α-L-rhamnosidases, keratinases, elastase, fibrinogenases, and fibrinogenogenases have been identified. Some of them are more active than enzymes isolated from microorganisms of terrestrial origin. Product Description popup.authors Abdulina Daryna R Avdiyuk Katerina V. Balko Olha I. Bamburа Оlga I. Borzova Nataliia V. Brovarska Oksana S Bulyhina Tetyana V. Harmasheva Inna L. Hyrenko Olha Holembiovsʹka Sofiya L. Grabova Hanna Yu. Gudzenko Olena V. Gulaeva Ganna B. Dolbik Nadia Zvinchuk Iryna Kireeva Viktoria Kushkina Alla I Luk'ianchuk Vitaliy Matselyukh Bohdan Pavlovych Nasikovska Oksana Pavlychenko Anna K. Polishchuk Lyudmyla V. Postoliuk Tetiana Sirchin Sergiy O. Chuiko Nelya V Yurieva Olena M. Yamborko Nadiya Anatoliivna popup.nrat_date 2023-02-22 Close