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Information × Registration Number 0223U002559, 0122U000659 , R & D reports Title Construction of biosensors and biofuel cells based on a combination of microbial cells - producers of oxidoreductases, purified enzymes and nanocomposite materials popup.stage_title Head Honchar Mykhailo V., д.б.н. Registration Date 23-02-2023 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2  Screening and synthesis of new nanozymes (NS) was carried out, combining the chemical reduction of inorganic salts of transition or noble metals with ascorbic acid with a new method of precipitation of NS on metal plates (Maple). The structural characterization of the obtained NPs was carried out using the methods of scanning electron, fluorescence, and atomic force microscopy; X-ray spectral analysis and dynamic light scattering. It was established that the size of the obtained materials is in the range from 15 nm (NS) to 15 μm (microzymes, MZ). The structural and morphological characteristics of the obtained HCF NPs were carried out using scanning electron microscopy, which confirmed the different shape of the NPs (elliptical, cubic and "nanoflower") and the difference in their sizes (from 50 nm to 5 μm). The catalytic properties of the obtained NPs in solution were studied. It was established that Cu/Ce/Aupl and Cupl have the highest peroxidase activity among the synthesized NZ. The kinetic parameters (KM and kcat) for the obtained "bare" HN and HN were determined. In peroxidase reactions, most of the synthesized NZ, in particular, Cu/Ce/Aupl, Cupl, Pt/Cu and Pd/Ce show the highest activity towards o-dianisidine. Synthesized NPs with reductase activity were used to design an amperometric biosensor for selenite analysis. The biosensor is characterized by high sensitivity (1700 A·M-1·m-2) and a wide linear range (from 16 μM to 1 mM) to introduced selenite. Product Description popup.authors popup.nrat_date 2023-02-23 Close