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Information × Registration Number 0223U002788, 0118U000041 , R & D reports Title Development of methods of genetic transformation and creation of a source material of winter wheat, resistant to drought popup.stage_title Head Mykhalska Svitlana I., Кандидат біологічних наук Registration Date 07-03-2023 Organization Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine popup.description2  Obtained biotechnological wheat plants into the genome of which, by Agrobacterium-mediated gene transfer, an additional copy of the ornithine aminotransferase gene of Medicago truncatula and plants with a double-stranded RNA suppressor of the proline dehydrogenase gene, which form two exons (located as an inverted repeat) and an intron of the proline dehydrogenase gene of Arabidopsis thaliana, were introduced. It was shown that in transgenic plants with a double-stranded RNA suppressor of the wheat proline dehydrogenase gene, the activity of the enzyme of proline catabolism is partially reduced, which is manifested in an increase in the content of free proline and an increase in resistance to osmotic stress. Biotechnological plants have the ability to germinate in conditions of water deficit created by the addition of the non-penetrating osmotic agent mannitol (0.5 M) and in conditions of sulfate salinity (1.2% Na2SO4). Seedlings of biotechnological wheat plants in such stressful conditions are ahead of the control plants by the main indicators of resistance to osmotic stress, biomass accumulation and height. It was shown that, under normal growing conditions, the T1 generation of wheat did not reliably differ from plants of the original form in terms of the main indicators of productivity Product Description popup.authors Bronnikova Larysa I. Komisarenko Alla H. Sergeeva Larysa Ye. popup.nrat_date 2023-03-07 Close