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Information × Registration Number 0223U003864, 0117U000385 , R & D reports Title Study of genetic determinants and new allele effects of genes to improve cereals under the negative impact of global climate change popup.stage_title Head Morhun Bohdan V, Registration Date 13-07-2023 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description2 The object of research was genetic determinants that determine resistance to biotic and abiotic factors, in particular, wheat transcription factors. The aim of the work was the study of new valuable genetic determinants in bread, hard and synthetic wheat lines that determine resistance to biotic and abiotic factors, and the development of systems for their molecular control. In the course of research, experiments were conducted using scientific equipment and apparatus of domestic and foreign production. Four DNA marker systems have been identified that will allow the researchers to effectively characterize wheat varieties under conditions of insufficient moisture. The structure of three genes of transcription factors (TaNAC2a, TaWRKY2, TaWRKY19) and dehydrin (Td29b) and their chromosomal localization were determined. The TaWRKY2 gene sequence was determined for 6 contrasting varieties of bread winter wheat. Amplification and sequencing of TaWRKY2-A1, TaWRKY2-B1, and TaWRKY2-D1 genes in four wheat varieties of the Eurasian growing zone were carried out. It was found that WRKY2 genes have a differential level of expression in drought conditions and in conditions of normal moisture availability. Amplification and sequencing of promoter regions of TaSHN1 genes from different subgenomes were carried out. It was established that lines of synthetic hexaploid wheat are carriers of the Ppo-A1a allele, which is responsible for the high activity of the polyphenol oxidase enzyme. DNA marker systems based on microsatellites (SSR and ISSR) were selected to study the genetic diversity of five species of the genus Crambe (C. maritima, C. aspera, C. tataria, C. steveniana, C. koktebelica). The genetic diversity of the most resistant higher plant Deschampsia antarctica was studied using the IRAP and REMAP marker systems. Product Description popup.authors Velikozon Ludmila Grigorivna Lakhneko Olha Romanivna Stepanenko Anton I. popup.nrat_date 2023-07-13 Close