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Information × Registration Number 0223U004703, 0120U104115 , R & D reports Title Development of new nanozymes as catalytic elements for enzymatic kits and chemo/biosensors popup.stage_title Head Gonchar Mykhailo V., Доктор біологічних наук Registration Date 30-11-2023 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2 Report on scientific research work: 314 pages, 158 draws, 61 tables, 6 appendices, 248 sources. NANOMATERIALS, NANOZYMS, MICROZYMES, CATALYTIC PARAMETERS, STRUCTURAL– FUNCTIONAL PROPERTIES, CATALYTIC CHARACTER, CHEMOENZYMATIC ANALYSIS ENZYMO– NANOZYMATIC METHOD The object of the research are synthetic nanomaterials (NM) with catalytic activity. Subject of the research – structural– functional and catalytic properties of NM. The purpose of the work is synthesis, study of structural, physico– chemical and catalytic properties of new catalytically active nanomaterials (nanozymes). Research methods: chemical, physico– chemical, biochemical, microbiological, enzymatic, microscopic methods (MM), X– ray spectral microanalysis, Infrared Fourier spectroscopy, dynamic light scattering (DLS). Results and novelty: For the first time: – the synthesis of new nanoparticles (NPs) of noble, transition and rare metals, as well as their hybrid forms, was carried out by the methods of chemical reduction of the corresponding salts and "green" synthesis, and their structural and morphological characteristics were carried out; – the analysis of the obtained nanomaterials for pseudoenzymatic activity (peroxidase, laccase, catalase and reductase) was carried out, and it was shown that among the 25 obtained NPs with different chemical composition, 10 have the highest peroxidase activity (1-8.6 Units/mg), 4 - laccase (0.8–4 Units/mg), one type each – catalase (0.3 Units/mg) and reductase (1.3 Units/mg) activity; – carbon - based nano/micromaterials (graphene, nanotubes, nanowalls, carbonized cellulose) were characterized using the methods of electron microscopy and positron annihilation spectroscopy, and it was shown that carbon materials have low peroxidase activity, which significantly increases after modification with hemin and noble metal nanoparticles (in 2– 15 times) compared to unmodified materials; – screening and structural– morphological characterization of magnetic NP Product Description popup.authors Mykhailo V. Gonchar Demkiv Olha M. Stasiuk Nataliia Ye. popup.nrat_date 2023-11-30 Close