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Information × Registration Number 0223U005261, 0119U100821 , R & D reports Title Endogenous factors for the development of myeloprpliferative tumors and prostate cancers popup.stage_title Head Telegeev Gennadiy D., Доктор біологічних наук Registration Date 19-12-2023 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  The subject of research: molecular mechanisms of BCR-ABL functioning, the role of Bcr domains in the functioning of normal cells and cells transformed by the BCR-ABL chimeric oncogene, the role of transcription factors in blast crisis, the development of drug resistance in prostate tumors. Methods: Standard molecular biological protocols, which included: isolation of RNA and DNA, oligonucleotide primers design, reverse transcriptase polymerase chain reaction (RT-PCR), PCR, real-time PCR, creation of recombinant DNA, Southern hybridization, DNA sequencing, fluorescence confocal microscopy of live and fixed mammalian cells, ultra-high-resolution fluorescence microscopy STED, etc. Work results. A hypothetical model is proposed according to which, during the formation of the BCR-ABL/GLG1 protein complex in the Golgi apparatus, the oncoprotein BCR/ABL deregulates the properties of GLG1 through phosphorylation, which causes disruption of downstream signaling pathways It was shown that Vit E suppresses the expression of the transcription factor Snail 1, the main inducer of EMT, in K562 culture, in contrast to the effect of imatinib, the drug of the first line of therapy. This creates prerequisites for the use of Vit E in CML therapy. Product Description popup.authors Antonenko Svitlana Vasylivna Gurianov Dmytro Sergiyovych Dybkov Mykhaylo Vasylovych Lysets'ka Tetyana Yuriyivna Shvachko Lyudmyla Pavlivna popup.nrat_date 2023-12-19 Close