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Information × Registration Number 0223U005600, 0120U104249 , R & D reports Title Advanced SERS-based nanoplatform for efficient detection of biomolecules and pathogens popup.stage_title Head Dzhahan Volodymyr M., Доктор фізико-математичних наук Registration Date 25-12-2023 Organization V. Lashkarev Institute of Semiconductor Physics of National Academy of Sciences of Ukraine popup.description2 A simple method of manufacturing effective SERS substrates with the morphology of inverted pyramids has been developed, which includes an original one-step synthesis of highly monodisperse SiO2 spheres at room temperature, their rapid self-assembly on a substrate and thermal evaporation of a silver layer several nanometers thick. The resulting SERS substrates are already quite effective when Raman spectra are excited by laser radiation with exc = 457 nm, but their efficiency can be significantly increased, and the resonant excitation can be shifted to exc = 532 nm by short-term annealing of SERS substrates in an argon atmosphere. The developed substrates allow easy detection of rhodamine 6G concentrations up to 10-13 M. The high efficiency of such SERS substrates for the detection of non-resonant analytes, in particular lysozyme and other biomolecules, which were functionalized from both biological buffers and aqueous solutions, was demonstrated. The high efficiency of the developed SERS substrates is achieved due to the fact that the proposed morphology contributes to the formation of numerous "hot spots" in the places of expected localization (enrichment) of the analyte during drying of the solvent - in the spaces between the SiO2 spheres - that is, in the tops of the inverted pyramids. Moreover, this morphology is likely to reduce the negative effects of thermophoresis, which can otherwise push the analyte away from “hot spots” and is rarely considered in the context of SERS. A new approach to formation of SERS substrates for multiplex detection has been developed, which consists in combining a set of separate plasmon nanostructures with different characteristics of morphology and spectral position of plasmon resonance. The fundamental possibility of achieving additional amplification of the Raman signal by adjusting the distance between plasmonic NPs, i.e. optimizing the configuration of "hot spots" by using an elastic substrate, has been demonstrated. Product Description popup.authors Isaieva Oksana F Buziashvili Anastasiia Yu. Hreshchuk Oleksandr M. Kapush Olga A. Mazur Nazar V. Pirko Yaroslav V. Sakharova Vladislava Oleksandr Y. Smirnov Yukhymchuk Volodymyr popup.nrat_date 2023-12-25 Close