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Registration Number

0224U001518, 0123U103772 , R & D reports

Title

Biophysical characteristics of circulating metastatic cells as potential targets of antimetastatic therapy

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Head

Dovbeshko Halyna I., д.ф.-м.н.

Registration Date

25-01-2024

Organization

Institute of Physics of National Academy of Sciences of Ukraine

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Subject of study: Lewis lung carcinoma cell cultures with high (LLC) and low (LLC/R9) metastatic potential. The purpose of the study: Determination of biophysical characteristics and experimental substantiation of the possibility of using these characteristics as potential therapeutic targets for antimetastatic therapy. Research methods and equipment: biochemical methods, cytofluorimetric method using labeled monoclonal antibodies, IR and Raman spectroscopy, confocal and CARS microscopy. Theoretical and practical results: IR and Raman spectroscopic markers of cells with different metastatic potential, namely, LLC and LLC/R9, with different methods of their cultivation - adhesive and non-adhesive - were determined. The differences found in the area of oscillations of CH3 and CH2 cells may be associated with changes in the structure and organization of their membranes, which correlates with structural changes in the lipid fraction isolated from these cells. Decreased intensity and high-frequency shift of PO2 bands associated with nucleic acid uptake correlate with decreased proliferative activity of non-adherent LLC growth cells. For LLC/R9, an increase in the intensity of the symmetric PO2 band was recorded, which correlates with a change in DNA packaging in the cell nucleus. Protein components have a predominant α-conformation in all samples, but in non-adhesive LLCs, the disordered protein phase is well registered. For LLC/R9, we registered a symmetrical band of Amid I with a significant shift of low frequencies indicating an increase in beta-phase or fibrillar proteins. This may be associated with changes in the cytoskeleton of the cell, mainly redistribution between the globular and fibrillar components of actin. Data from microscopic studies correlate with data from IR spectroscopy. Novelty: For the first time, in vitro studies of tumor cells of the same origin, but with different metastatic potential, were carried out.

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Yesylevskyy Semen Oleksandrovych
Barabash Yurii M.
Gnayuk Olena P
Dovbeshko Halyna I.
Karakhim Sergii O
Kolesnyk Denys L.
Olenchuk Maryna Volodymyrivna
Solyanik Galyna I.
Stepanov Yurii V.
Shevliakova Hanna Viktorivna
Yurchenko Olga V.

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2024-01-25