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Information × Registration Number 0224U001588, 0123U101081 , R & D reports Title Synthesis of recombinant pharmaceutical proteins and increasing the content of biologically active natural compounds in plants popup.stage_title Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 25-01-2024 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description2  During the implementation of the project, the antibacterial activity of extracts of transgenic lettuce (Lactuca sativa L.) and mizuna (Brassica rapa subsp. nipposinica var. laciniata) plants expressing colicin M against two main enterohemorrhagic strains of E. coli (EHEC): O157: H7 and O104:H4, as well as multiresistant E. coli strains resistant to beta-lactam antibiotics and carbapenems was confirmed. It was shown that the amount of colicin M in extracts of transgenic lettuce and mizuna plants varies from 1.06 to 3.36 μg/ml and these values are significantly higher than the minimum inhibitory concentration (MIC) of purified recombinant colicin M for the tested pathogenic bacterial strains. These results indicate the possibility of using the obtained edible transgenic plants accumulating colicin M as antimicrobial drugs to control the spread of pathogenic E. coli strains. Techniques for transient expression of recombinant proteins in sweet basil (Ocimum basilicum) and duckweed (Lemnaceae) plants were also improved, and a protocol that provides a basis for cryopreservation of duckweed germplasm collections, saving labor for in vitro cultivation and saving reference samples was developed. The presence of a stimulating effect of pre-sowing irradiation of medicinal chamomile seeds of different varieties in the range of doses from 5 to 20 Gy on the accumulation of pharmaceutically valuable secondary metabolites was proven. The obtained dose dependences indicate the possibility of the influence of acute X-ray irradiation of seeds on the increase in the concentration of phenols in medicinal raw materials. A protocol for microclonal reproduction and adaptation to soil conditions of Bletilla plants (B. striata), a medicinal plant with hemostatic properties, was developed. The presence of fibrinogenase activity in B. striata leaf extracts was also confirmed, which is a promising direction for further research. Product Description popup.authors Belokurova Valeriia B. Bulko Olga V. Halych Taras V. Zhuk Vladyslav V. Litvinov Sergiy V. Lystvan Kateryna V. Lyoshyna Lyidmyla H Matvieieva Nadiya A. Ovcharenko Olga O Peterson Anton A. Pchelovska Svitlana A. Rudas Volodymyr A. Sindarovska Iana R Sakada Volodymyr I. Symonenko Yurij V. Shylina Yuliia V. Shcherbak Nataliіa L popup.nrat_date 2024-01-25 Close