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Information × Registration Number 0224U002475, 0119U001485 , R & D reports Title Biotechnological potential of microorganisms from natural and anthropogenics ecosystems popup.stage_title Head Registration Date 15-02-2024 Organization National university of food technologies popup.description2  Report: 141 pages, 3 hours, 27 tables, 15 figures. ETAPOLAN, ACINETOBACTER SP., SURFACTANTS, NOCARDIA VACCINII, NANOPARTICLES, BIOGENIC SYNTHESIS The possibility of intensification of the synthesis of microbial polysaccharide stagepolan on a mixture of energetically surplus substrates has been established. Technologies for the production of stagepolan on a mixture of spent sunflower oil and C2-C6 substrates (acetate, ethanol, and molasses) have been developed, which include: 1) growth of the inoculum on a monosubstrate of spent oil, 2) use of potassium nitrate as a source of nitrogen, 3) increasing the content of Mg2+ in the culture medium to 5 mM, and 4) fractional introduction of substrates. The implementation of such approaches has made it possible to increase the synthesis of etapolaan to 16-18 g/l. The possibility of regulating the biological activity of surface-active substances of N. vaccinii IMV B-7405 by increasing the concentrations of calcium, sodium, and potassium cations in the culture medium, as well as by introducing competitive microorganisms into the medium, was established. Surface-active substances of N. vaccinii IMV B-7405 showed synergism in the destruction of yeast and bacterial biofilms on silicone tubes in a mixture with nystatin, fluconazole, and tea tree essential oil (a 2-3-fold increase in the degree of destruction of biofilms compared to the use of monopreparations), which gives it is possible to consider them as promising components of "antifungal" and "antibacterial locks.” Using S. cerevisiae M437 and S. cerevisiae 71 B, it was possible to carry out the biosynthesis of AgNPs. AgNPs were synthesized in the supernatant of the culture liquid and cell-free extract of S. cerevisiae M437 by adding silver nitrate directly to these solutions. AgNPs, which were obtained by introducing silver nitrate into the culture liquid of S. cerevisiae 71 B, showed antibacterial and anti-adhesive activity against the reference strains S. aureus ATCC 25923 Product Description popup.authors Voronenko Andrii A. Kliuchka Lilia V. Paliichuk Olesia I. Pyroh Tetiana P. Skrotska Oksana I. Stabnikov Viktor P. Udymovych Viktor M. Kharchenko Yevhen V. popup.nrat_date 2024-02-15 Close