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Information × Registration Number 0224U002590, 0122U000659 , R & D reports Title Construction of biosensors and biofuel cells based on a combination of microbial cells - producers of oxidoreductases, purified enzymes and nanocomposite materials popup.stage_title Head Honchar Mykhailo V., д.б.н. Registration Date 20-02-2024 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2  Scientific Report: 81 p., 30 figures, 14 tables, 63 references. Amperometric biosensors (ABS) based on various oxidoreductases, nCuCe nanozyme and porous gold (porAu) have been constructed. Bionanocomposite electrodes are used for assay, glucose, primary alcohols, methylamine, L-arginine, and catechol. The created monoenzyme ABS have improved analytical characteristics compared to the corresponding bienzyme analogues containing natural horseradish peroxidase. It was found that after modifying the surface of the nCuCe graphite electrode, the sensitivity of ABS increases from 4 to 18 times (for methylamine oxidase /nCuCe/porAu/GE). From the extract of the cap fungus Amanita phalloides by chromatographic purification, a 1600-fold purified (compared to the original extract) preparation of arginine oxidase (ArgO) with a specific activity of 0.56 U/mg of protein was obtained. The main physicochemical and kinetic properties of the enzyme preparation were studied, namely, the molecular weight of the subunit, pH and temperature optimums, stability and substrate specificity, kinetic parameters relative to L-arginine (Arg); Compounds that have a stabilizing and inhibitory effect on the enzyme have been identified. Sensitive and selective ABS for quantitative analysis of Arg using ArgO in combination with new synthesized ammonium-selective metal-hybrid nanomaterials based on nCu and nCdCu have been developed. The created ABS are characterized by good selectivity and high sensitivity to Arg (1700 and 2300 A• M-1•m-2 for nCdCu and nCu, respectively), as well as satisfactory operational stability. New ABS were constructed on the basis of nanoparticles (nPt, nAu, nAu/Pt) and flavocytochrome b2 electrodeposited on the surface of a graphite electrode. The biosensor with FC b2/nAu/nPtAu/GE architecture detects the highest sensitivity to lactate (3750 A• M−1•m−2). The best-performing ABS has been successfully used for the analysis of lactate in food products (using yogurt as an exa Product Description popup.authors Gayda Galyna Z. Honchar Mykhailo V. Horecha Mariia V. Demkiv Olha M. Zakalska Oksana M. Zakalskyi Andrii Ye. Moroz Anna A. Serkiz Roman Ya. Stasiuk Nataliia Ye. popup.nrat_date 2024-02-20 Close