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Information × Registration Number 0224U003130, 0118U000451 , R & D reports Title Investigation of functional sites of cytoplasmic membrane of plant cells under real and simulated microgravity conditions popup.stage_title Head Kordyum Yelizaveta L, чл.-кор. НАНУ Registration Date 10-04-2024 Organization M.G.Kholodny Institute of Botany NASU popup.description2  Elucidating the degree of sensitivity of cells to gravity and microgravity provides key information required for the creation of bioregenerative life support systems, of which plants are a necessary component as sources of oxygen, clean water and food for astronauts in long space flights and visiting planets. In the light of the tasks of modern comic biology, the goal of the project is to experimentally test the idea that the gravisensitivity of microdomains of the cytoplasmic membrane - "lipid rafts" - of plant cells plays a key role in changes in membrane permeability and the activity of membrane-bound structural proteins and enzymes in the process of plant adaptation to conditions microgravity. It is suggested that cholesterol- and sphingolipid-enriched rafts modulate protein interactions and are thus involved in numerous vital cellular processes. Pea seedlings, which will be grown for 3 and 6 days in stationary conditions and conditions of simulated microgravity, were chosen as research objects. Simulations of microgravity in ground experiments will be created using slow horizontal or randomly rotating clinostats (Random Positioning Machine, RPM), which partially reproduce the biological effects of microgravity in spaceflight. As is well known, it is ground-based research that leads to space experiments, they have always been the basis for the development and concretization of hypotheses that are later tested in space flight. The fraction of the cytoplasmic membrane (CM) is isolated from the roots of pea seedlings, and the fraction of detergent-resistant microdomains of the membrane (lipid rafts) is isolated from the CM fraction, which serves as the object of further research using the methods of light, electron and confocal microscopy, biochemistry and molecular biology Product Description popup.authors Artemenko Olha A. Brykov Vasyl O. Ovcharenko Yuliia V. popup.nrat_date 2024-04-10 Close