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Information × Registration Number 0224U031470, 0122U001762 , R & D reports Title "Study of the phenomenon of biofilm formation and antibiotic resistance in microorganisms for the development of molecular genetic means for the diagnosis of emergent zoonoses" popup.stage_title Head Ushkalov Valerii O., к.вет.н. Registration Date 30-04-2024 Organization National University of Life and Environmental Sciences of Ukraine popup.description2  The object of research is the causative agents of emergent zoonoses. The subject of the study is determinants of pathogenicity of zoonotic agents and methods of their diagnosis. The purpose of the work is experimental substantiation of the use of genetic factors of pathogenicity in the creation of diagnostic tools for emerging zoonoses. Research methods - modern microbiological methods (bacteriological studies of biological material on nutrient media, determination of the nature of vegetation, colony morphology - size, shape, consistency, surface character, pigmentation, etc., production of smears, their staining and microscopy, study of enzymatic properties), serological ( RA), molecular genetic (identification and typing of microorganism isolates in PCR), mathematical and statistical (processing of the received data using the Microsoft Excel 2007 application package). The research was aimed at studying modern scientific data on the control of microorganisms that cause zoonoses. The study of the risks of food products of animal and plant origin, which are contaminated with pathogenic microorganisms, was carried out. An analysis of modern requirements for the diagnosis of the specified diseases and recommendations regarding the diagnosis and identification of pathogens using bacteriological, molecular genetic studies and the development of molecular genetic indication tools using PCR of zoonotic pathogens was carried out. Cultures from the genus Bacillus and the genus Lactobacillus were isolated from litter samples of clinically healthy chickens. The investigated isolates formed biofilms of high density, showed an antagonistic effect in relation to pathogenic microorganisms and resistance to the aggressive environment of the digestive tract, which became the basis for the selection of probiotic cultures. The method of production of probiotic "SporoLex" is experimentally substantiated. A library of genes ensuring the pathogenicity of microorganisms has been created. Product Description popup.authors Ishchenko Liudmyla V Bezpalko Oleksandr O. Boianovskyi Serhii O. Vishovan Yurii Yu. Vyhovska Liliia M. Davulovska Liliana O. Danchuk Viacheslav V. Lytvynenko Viktor М. Mazur Tetiana V. Masliuk Alla V. Machuskyi Oleksandr V Melnyk Volodymyr V Nedosiekov Vitalii V. Starodub Mykola F. Ushkalov Artem V. popup.nrat_date 2024-04-30 Close