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Information × Registration Number 0224U031742, 0119U100475 , R & D reports Title Development of universal multistage biotechnology "in vitro - ex vitro - in situ" to stabilize populations of rare plant species popup.stage_title Head Drobyk Nadiia M., Доктор біологічних наук Registration Date 10-06-2024 Organization Ternopil Volodymyr Hnatiuk National Pedagogical University popup.description2  Object involved the adaptation of microclonal propagated in vitro plants of rare species of Ukrainian flora to ex vitro and in situ conditions. Subject: structural and functional transformations of biotechnological plants of rare species of the genera Gentiana L. and Carlina L. during in vitro cultivation and adaptation to ex vitro and in situ conditions.Aim: develop a universal multi-stage technology for adapting planting material of rare plants grown under artificial conditions to ex vitro and in situ conditions based on studies of changes in their physiological and biotechnological characteristics during the optimization of cultivation conditions. Methods: in vitro cultivation; anatomical leaf structure studies (microscopic measurements have been conducted using an eyepiece micrometer on the XSP-146TR microscope and ImageJ program); spectrophotometric method using the SF-46 spectrophotometer; biophysical methods, namely chlorophyll fluorescence induction (CFI) using the MultispeQ fluorometer (MSU, USA) and the online platform PhotosynQ (USA); biometric methods (for determining leaf area, the mobile application Petiole and its companion OpenCV Manager have been used); statistical methods, specifically ANOVA (Tukey test) and principal component analysis (PCA). The application of a systematic approach allowed the development of an effective multi-stage technology for adapting in vitro plants to both ex vitro and in situ conditions,that has been confirmed by 100% survival of plants of general Gentiana and Carlina at the end of the first growing season under in situ conditions. By the end of the second growing season, the survival rate of plants was 61–70%, remained unchanged. New universal system of marker criteria has been suggested for assessing the structural and functional state of biotechnologically derived plants in vitro and ex vitro, allowing the selection of planting material with high adaptive potential for in situ conditions at all stages of cultivation. Product Description popup.authors Herts Andrii I. Hrytsak Liudmyla R. Humeniuk Halyna B. Kolisnyk Khrystyna M. Kravets Nataliia B. Maiorova Oksana Yu. Prokopiak (Mosula) Mariana Z. popup.nrat_date 2024-06-10 Close