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Information × Registration Number 0224U032517, (0124U003810) , R & D reports Title Conducting research on optimizing the cultivation conditions of Bacillus subtilis IMB B-7521 culture popup.stage_title Проведення досліджень щодо оптимізації умов культивування культури Bacillus subtilis IMB B-7521 Head Nudha Andrii Yu., Registration Date 08-11-2024 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 Conducting research on optimizing the cultivation conditions of Bacillus subtilis IMB B-7521 culture popup.description2  Research object: Microorganisms Bacillus subtilis IMB B-7521. Possibilities of working out the fermentation process. Purpose of work: Conduct research and find optimal parameters for cultivation, concentration and storage of microorganisms Bacillus subtilis IMB B-7521. Research methods: microbiological, physicochemical and biochemical. The scientific and technical work carried out included: - research and optimization of all necessary physiological and biochemical parameters, including selection of seed material, identification of their nutritional needs, growth properties of the studied strain of bacteria; - determining the possibility of concentrating the culture liquid using a flow ultracentrifuge - preservation of biological activity of the producer. It is established that: The main technological parameters can be reproduced in the conditions of industrial fermentation. That is, it is possible to carry out an effective process of fermentation of the microorganism and obtain a culture liquid with a high titer of colony-forming units. It has been found and practically proven that the optimal cultivation parameters for the strain are a cultivation temperature of 24°C with a pH range of 6.5 – 7.4 and aeration of 0.2 vol. pov\1 vol. cult. family. Cultivation must be carried out within 36-48 hours. A practical check was carried out on the possibility of using flow-type ultracentrifuge 102 K - o1 for the concentration of culture liquid. Product Description popup.authors Ananko Yevhen F. Dymydenko Valentyna P. Nudha Andrii Yu. Smyrnova Halyna F. popup.nrat_date 2024-11-08 Close